Agradecimentos: Mohsen Gavahian would like to thank the support of Ministry of Economic Affairs, project no. 107-EC-17-A-22-0332 and 108-EC-17-A-22-0332, Taiwan, Republic of China. Work in the laboratory of Sotirios C. Kampranis is supported by the Novo Nordisk Foundation (grants: NNF16OC0021760,...

Agradecimentos: Mohsen Gavahian would like to thank the support of Ministry of Economic Affairs, project no. 107-EC-17-A-22-0332 and 108-EC-17-A-22-0332, Taiwan, Republic of China. Work in the laboratory of Sotirios C. Kampranis is supported by the Novo Nordisk Foundation (grants: NNF16OC0021760, NNF17OC0027646, NNF18OC0034784, NNF18OC0031872, NNF16OC0019554) and the Independent Research Fund Denmark (grants: DFF-7017-00275 and 8022-00254B)

Abstract: The recent progress in genetic engineering has brought multiple benefits to the food and agricultural industry by enhancing the essential characteristics of agronomic traits. Powerful tools in the field of genome editing, such as siRNA-mediated RNA interference for targeted suppression of...

Abstract: The recent progress in genetic engineering has brought multiple benefits to the food and agricultural industry by enhancing the essential characteristics of agronomic traits. Powerful tools in the field of genome editing, such as siRNA-mediated RNA interference for targeted suppression of gene expression and transcription activator-like effector nucleases (TALENs) and zinc-finger nucleases (ZFNs) for DNA repair have been widely used for commercial purposes. However, in the last few years, the discovery of the CRISPR-Cas9 system has revolutionized genome editing and has attracted attention as a powerful tool for several industrial applications. Herein, we review current progresses in the utilization of the CRISPR-Cas9 system in the food and agricultural industry, particularly in the development of resistant crops with improved quality and productivity. We compare the CRISPR system with the TALEN and ZFN nucleases–based methods and highlight potential advantages and shortcomings. In addition, we explore the state of the global market and discuss the safety and ethical concerns associated with the application of this technology in the food and agricultural industry

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