Agradecimentos: We thank the Brazilian Nanotechnology National Laboratory (LNNano, CNPEM) and the Analytical Resources and Calibration Laboratory (LRAC) from School of Chemical Engineering (Unicamp) for providing the analytical facilities. We also thank Elisabeth Shaw for her excellent assistance...

Agradecimentos: We thank the Brazilian Nanotechnology National Laboratory (LNNano, CNPEM) and the Analytical Resources and Calibration Laboratory (LRAC) from School of Chemical Engineering (Unicamp) for providing the analytical facilities. We also thank Elisabeth Shaw for her excellent assistance during the XPS analyses at the CMSE-MIT shared facilities. We acknowledge CNPq and São Paulo Research Foundation (FAPESP, grant n. 2013/05135-1 grant n. 2016/10193-9 and n. 2016/19976-6) for the financial support to conduct this project. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001 (Procad 88882.151600/2017-01 and PNPD/FEQ)

Immobilization of cells on surfaces can be strongly affected by several properties of a substrate, including surface properties, topography and charge density. Controlling the cell adhesion mechanism is a real challenge in biotechnological processes. Here, we assessed the influence of the average...

Immobilization of cells on surfaces can be strongly affected by several properties of a substrate, including surface properties, topography and charge density. Controlling the cell adhesion mechanism is a real challenge in biotechnological processes. Here, we assessed the influence of the average degree of deacetylation of chitosan (DD) and the polyelectrolyte solution pH on the physicochemical properties of multilayered hyaluronan/chitosan films and their ability to bind tumor cells. These functional coatings explore CD44-hyaluronate interactions to improve the capture of tumor cells (PC3 line). Film characterization was carried out using XPS, UV-Vis and scanning probe techniques such as Capacitance, KPFM and AFM-IR measurements. The increase in the solution pH from 3.0 to 5.0 was the major factor to control both the film structure and the cell capture, enhancing the number of cells adhered in a range of 1.5 to 3.5-fold. These results also suggest that limited HA availability induces cell-film adhesion, whereas more irregular and rougher surfaces inhibit this process. These findings indicate that surface chemistry and morphology are critical factors for the development of new biomaterials designed to capture cells selectively

FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP

2013/05135-1; 2016/10193-9; 2016/19976-6

CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ

COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES

88882.151600/2017-01

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