Association between cationic liposomes and low molecular weight hyaluronic acid
Antonio A. M. Gasperini, Ximena E. Puentes-Martinez, Tiago Albertini Balbino, Thais de Paula Rigoletto, Gabriela de Sá Cavalcanti Corrêa, Alexandre Cassago, Rodrigo Villares Portugal, Lucimara Gaziola de La Torre, Leide P. Cavalcanti
ARTIGO
Inglês
This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). The cationic...
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This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). The cationic liposome/HA complexes were evaluated to determine their mesoscopic structure, average size, zeta potential, and morphology as a function of the amount of HA in the system. Small angle X-ray scattering results revealed that neighboring cationic liposomes either stick together after a partial coating of low concentration HA or disperse completely in excess of HA, but they never assemble as multilamellar vesicles. Cryo-transmission electron microscopy images confirm the existence of unilamellar vesicles and large aggregates of unilamellar vesicles for HA fractions up to 80% (w/w). High concentrations of HA (> 20% w/w) proved to be efficient for coating extruded liposomes, leading to particle complexes with sizes in the nanoscale range and a negative zeta potential
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2011/19952-6; 2010/02203-8
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
400796/2012-0
Fechado
DOI: https://doi.org/10.1021/la5045865
Texto completo: https://pubs.acs.org/doi/10.1021/la5045865
Association between cationic liposomes and low molecular weight hyaluronic acid
Antonio A. M. Gasperini, Ximena E. Puentes-Martinez, Tiago Albertini Balbino, Thais de Paula Rigoletto, Gabriela de Sá Cavalcanti Corrêa, Alexandre Cassago, Rodrigo Villares Portugal, Lucimara Gaziola de La Torre, Leide P. Cavalcanti
Association between cationic liposomes and low molecular weight hyaluronic acid
Antonio A. M. Gasperini, Ximena E. Puentes-Martinez, Tiago Albertini Balbino, Thais de Paula Rigoletto, Gabriela de Sá Cavalcanti Corrêa, Alexandre Cassago, Rodrigo Villares Portugal, Lucimara Gaziola de La Torre, Leide P. Cavalcanti
Fontes
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Langmuir: the ACS journal of surfaces and colloids Vol. 31, no. 11 (2015), p. 3308-3317 |