High-throughput continuous production of liposomes using hydrodynamic flow-focusing microfluidic devices
ARTIGO
Inglês
The microfluidic hydrodynamic flow-focusing is a simple technique for nanoscale liposome formation that provides several advantages compared to the traditional manufacturing techniques. This work aimed to perform a systematic study of the liposome formation using planar microfluidic devices with...
The microfluidic hydrodynamic flow-focusing is a simple technique for nanoscale liposome formation that provides several advantages compared to the traditional manufacturing techniques. This work aimed to perform a systematic study of the liposome formation using planar microfluidic devices with different channel aspect-ratios, as an alternative to enhance the throughput of liposome synthesis. In general, liposomes with a low polydispersity and a precise control of the size were successfully produced from alteration of the flow rate ratio and channel aspect-ratio. The higher aspect-ratio enabled the most rapid generation of liposomes with similar diameter and significant lower polydispersity index than the obtained by other batch technique. Besides, β-carotene was successfully incorporated into liposomes with efficiency of approximately 60% and the incorporation ability was not specific to a choice of microfluidic device aspect-ratio. The results suggest that the use of microfluidic devices could be employed for liposome production with a possible advantage to minimize the degree of parallelization of processes. These results demonstrate the potential technical feasibility of microfluidic processes for future industrial applications
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2009/54137-1; 2007/54520-4
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
140283/2013-7; 140270/2014-0; 140271/2014-7; 305477/2012-9
Fechado
High-throughput continuous production of liposomes using hydrodynamic flow-focusing microfluidic devices
High-throughput continuous production of liposomes using hydrodynamic flow-focusing microfluidic devices
Fontes
Colloids and surfaces. B: biointerfaces Vol. 156 (2017), p. 349-357 |