Ethanol precipitation as a downstream processing step for concentration of xylanases produced by submerged and solid-state fermentation
Max A. L. Costa, Cristiane S. Farinas, Everson A. Miranda
ARTIGO
Inglês
Agradecimentos: The authors thank the Brazilian institutions CAPES, FAPESP, CNPq, and EMBRAPA for financial support
Xylanases have applications in different industries, being produced by microbial cultivations using submerged (SmF) or solid-state fermentation (SSF). Precipitation stands out as a potential method for the concentration of xylanases, especially with the use as ethanol as the precipitant due to its...
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Xylanases have applications in different industries, being produced by microbial cultivations using submerged (SmF) or solid-state fermentation (SSF). Precipitation stands out as a potential method for the concentration of xylanases, especially with the use as ethanol as the precipitant due to its compatibility with the biorefinery concept. This paper presents a comparative laboratory scale study of ethanol precipitation of xylanases produced by Aspergillus niger cultivated under SSF and SmF. Precipitation conditions were selected according to a central composite design. Statistical analysis showed a significant effect of pH on the recoveries of total protein and xylanase activity. The kinetic profiles showed that a relatively short period of time (up to 15 min) was sufficient to recover most of the xylanase activity precipitated under the selected conditions. Xylanase recoveries of 65 and 79% were achieved for the SSF and SmF enzymatic complexes, respectively
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CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
Aberto
Ethanol precipitation as a downstream processing step for concentration of xylanases produced by submerged and solid-state fermentation
Max A. L. Costa, Cristiane S. Farinas, Everson A. Miranda
Ethanol precipitation as a downstream processing step for concentration of xylanases produced by submerged and solid-state fermentation
Max A. L. Costa, Cristiane S. Farinas, Everson A. Miranda
Fontes
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Brazilian journal of chemical engineering Vol. 35, no. 2 (Apr./June, 2018), p. 477-488 |