The Curauá (Ananaserectifolius LB Smith) is a species belonging to the family Bromeliaceae. Occurs in the states of Para, Amazonas, Amapá, Acre, Mato Grosso and Goiás, has two varieties, purple and white, and its fibers are used in automotive and textile industries due to its strength, softness and...

The Curauá (Ananaserectifolius LB Smith) is a species belonging to the family Bromeliaceae. Occurs in the states of Para, Amazonas, Amapá, Acre, Mato Grosso and Goiás, has two varieties, purple and white, and its fibers are used in automotive and textile industries due to its strength, softness and lightweight. Currently, only the fiber is used in industry, the rest is considered waste. However, this residue contains compounds with important properties to be discovered and studied. An enzyme complex found in this residue is bromelain, a group of proteolysis’ enzymes with application in several areas such as in the food, pharmaceutical and cosmetics industries. Today, its use is aiming the pharmaceutical industry, the production of ointments, gels, creams and lotions because they offer a wide range of therapeutic efficacies: antiedemas, anti-inflammatory, antithrombotic and fibrinolytic activities. This work studied the enzyme purification and recovery presented in the leaves of Curauá by means of an ATPS (aqueous two-phase system) PEG 4000/potassium phosphate. The protein content was measured by Bradford reagent and the enzymatic activity was measured by the Biuret reagent. Batch assays were performed aiming the enzyme extraction and recuperation, using the partition coefficient as indicator. It was used pH 7.0, 8.0 and 9.0, varying the proportional composition between the polymeric and the saline phases (tie-lines). The white variety of Curauá was used. The best purification factor was 4.53 and about 113.04 µg/mL of total protein measured by Bradford

FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP

COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES

CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ

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