Effect of the initial concentration on the equilibrium liquid phase concentration at salting out of proteins
Moyses Batista de Araujo Junior, Pedro de Alcantara Pessoa Filho, Everson Alves Miranda
ARTIGO
Inglês
Background Protein precipitation is a unit operation commonly employed in bioprocesses. The most important thermodynamic property for designing an industrial precipitation operation is the solubility. The solubility is defined as the protein concentration in a certain liquid phase in equilibrium...
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Background Protein precipitation is a unit operation commonly employed in bioprocesses. The most important thermodynamic property for designing an industrial precipitation operation is the solubility. The solubility is defined as the protein concentration in a certain liquid phase in equilibrium with its solid precipitate. In some cases, the protein concentration in the liquid phase at equilibrium depends on the initial concentration of the protein, therefore being an apparent solubility. Here an assessment of the effect of the molecular mass of proteins on the dependence of the apparent solubility on the initial protein concentration is reported. RESULTS Proteins with different molecular masses (bovine insulin, 5.7 kDa; chicken egg white lysozyme, 14.7 kDa; porcine trypsin, 23.4 kDa; bovine serum albumin (BSA), 66.0 kDa) at different initial concentrations were precipitated with NaCl at constant pH and temperature. Lysozyme was the only protein that showed no change in its solubility as a function of the initial protein concentration at the studied conditions. For insulin, the effect of the initial concentration on the apparent solubility was verified only at low salt concentrations. The apparent solubility of proteins with higher molecular mass, i.e. trypsin and albumin, showed dependence on the initial protein concentration: the higher the initial protein concentration, the higher the apparent solubility. CONCLUSION Based on the data of this work and the literature, the molecular mass appears to affect the dependence of the apparent solubility of proteins on their initial concentration in precipitation processes. (c) 2019 Society of Chemical Industry
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CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
001
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
Fechado
Effect of the initial concentration on the equilibrium liquid phase concentration at salting out of proteins
Moyses Batista de Araujo Junior, Pedro de Alcantara Pessoa Filho, Everson Alves Miranda
Effect of the initial concentration on the equilibrium liquid phase concentration at salting out of proteins
Moyses Batista de Araujo Junior, Pedro de Alcantara Pessoa Filho, Everson Alves Miranda
Fontes
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Journal of Chemical Technology and Biotechnology Vol. 94, no. 11 (Nov., 2019), p. 3706-3712 |