Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail
ARTIGO
Inglês
The aim of this work was to study cocktail supplementation for sugar cane bagasse hydrolysis, where the enzymes were provided from both commercial source and microorganism cultivation (Trichoderma reesei and genetically modified Escherichia coli), followed by purification. Experimental simplex...
The aim of this work was to study cocktail supplementation for sugar cane bagasse hydrolysis, where the enzymes were provided from both commercial source and microorganism cultivation (Trichoderma reesei and genetically modified Escherichia coli), followed by purification. Experimental simplex lattice mixture design was performed to optimize the enzymatic proportion. The response was evaluated through hydrolysis microassays validated here. The optimized enzyme mixture, comprised of T. reesei fraction (80%), endoglucanase (10%) and β-glucosidase (10%), converted, theoretically, 72% of cellulose present in hydrothermally pretreated bagasse, whereas commercial Celluclast 1.5 L converts 49.11% ± 0.49. Thus, a rational enzyme mixture designed by using synergism concept and statistical analysis was capable of improving biomass saccharification
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2008/57873-8; 2010/08089-2; 2012/23223-2
Fechado
Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail
Improvement on sugar cane bagasse hydrolysis using enzymatic mixture designed cocktail
Fontes
Bioresource technology Vol. 187 (July, 2015), p. 173-181 |