Protective efficacy of different strategies employing Mycobacterium leprae heat-shock protein 65 against tuberculosis
Patrícia R M Souza, Carlos R Zárate-Bladés, Juliana I Hori, Simone G Ramos, Deison S Lima, Tatiana Schneider, Rogério S Rosada, Lucimara G L Torre, Maria Helena A Santana, Izaíra T Brandão, Ana P Masson, Arlete A M Coelho-Castelo, Vânia L Bonato, Fabio C S Galetti, Eduardo D Gonçalves, Domingos A...
Patrícia R M Souza, Carlos R Zárate-Bladés, Juliana I Hori, Simone G Ramos, Deison S Lima, Tatiana Schneider, Rogério S Rosada, Lucimara G L Torre, Maria Helena A Santana, Izaíra T Brandão, Ana P Masson, Arlete A M Coelho-Castelo, Vânia L Bonato, Fabio C S Galetti, Eduardo D Gonçalves, Domingos A Botte, Jeanne B M Machado, Celio L Silva
ARTIGO
Inglês
Tuberculosis is a major threat to human health. The high disease burden remains unaffected and the appearance of extremely drug-resistant strains in different parts of the world argues in favor of the urgent need for a new effective vaccine. One of the promising candidates is heat-shock protein 65...
Tuberculosis is a major threat to human health. The high disease burden remains unaffected and the appearance of extremely drug-resistant strains in different parts of the world argues in favor of the urgent need for a new effective vaccine. One of the promising candidates is heat-shock protein 65 when used as a genetic vaccine (DNAhsp65). Nonetheless, there are substantial data indicating that BCG, the only available anti-TB vaccine for clinical use, provides other important beneficial effects in immunized infants. Methods: We compared the protective efficacy of BCG and Hsp65 antigens in mice using different strategies: i) BCG, single dose subcutaneously; ii) naked DNAhsp65, four doses, intramuscularly; iii) liposomes containing DNAhsp65, single dose, intranasally; iv) microspheres containing DNAhsp65 or rHsp65, single dose, intramuscularly; and v) prime–boost with subcutaneous BCG and intramuscular DNAhsp65. Results: All the immunization protocols were able to protect mice against infection, with special benefits provided by DNAhsp65 in liposomes and prime–boost strategies. Conclusion: Among the immunization protocols tested, liposomes containing DNAhsp65 represent the most promising strategy for the development of a new anti-TB vaccine
Fechado
Protective efficacy of different strategies employing Mycobacterium leprae heat-shock protein 65 against tuberculosis
Patrícia R M Souza, Carlos R Zárate-Bladés, Juliana I Hori, Simone G Ramos, Deison S Lima, Tatiana Schneider, Rogério S Rosada, Lucimara G L Torre, Maria Helena A Santana, Izaíra T Brandão, Ana P Masson, Arlete A M Coelho-Castelo, Vânia L Bonato, Fabio C S Galetti, Eduardo D Gonçalves, Domingos A...
Patrícia R M Souza, Carlos R Zárate-Bladés, Juliana I Hori, Simone G Ramos, Deison S Lima, Tatiana Schneider, Rogério S Rosada, Lucimara G L Torre, Maria Helena A Santana, Izaíra T Brandão, Ana P Masson, Arlete A M Coelho-Castelo, Vânia L Bonato, Fabio C S Galetti, Eduardo D Gonçalves, Domingos A Botte, Jeanne B M Machado, Celio L Silva
Protective efficacy of different strategies employing Mycobacterium leprae heat-shock protein 65 against tuberculosis
Patrícia R M Souza, Carlos R Zárate-Bladés, Juliana I Hori, Simone G Ramos, Deison S Lima, Tatiana Schneider, Rogério S Rosada, Lucimara G L Torre, Maria Helena A Santana, Izaíra T Brandão, Ana P Masson, Arlete A M Coelho-Castelo, Vânia L Bonato, Fabio C S Galetti, Eduardo D Gonçalves, Domingos A...
Patrícia R M Souza, Carlos R Zárate-Bladés, Juliana I Hori, Simone G Ramos, Deison S Lima, Tatiana Schneider, Rogério S Rosada, Lucimara G L Torre, Maria Helena A Santana, Izaíra T Brandão, Ana P Masson, Arlete A M Coelho-Castelo, Vânia L Bonato, Fabio C S Galetti, Eduardo D Gonçalves, Domingos A Botte, Jeanne B M Machado, Celio L Silva
Fontes
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Expert opinion on biological therapy Vol. 8, no. 9 (2008), p. 1255-1264 |