Agradecimentos: The authors are grateful for support from the NIH (STTRR41GM116530) to J.O.N., J.Y.W., and R.G.H. and fromBoston University’s College of Engineering Dean’s CatalystAward to R.G.H. and J.Y.W. We thank A. Stucchi for helpfuland stimulating discussions and V. Doheny for evaluating...

Agradecimentos: The authors are grateful for support from the NIH (STTRR41GM116530) to J.O.N., J.Y.W., and R.G.H. and fromBoston University’s College of Engineering Dean’s CatalystAward to R.G.H. and J.Y.W. We thank A. Stucchi for helpfuland stimulating discussions and V. Doheny for evaluating theTerason parameters used in US experiments. C.A.G. acknowl-edges support from an NSF Graduate Research Fellowshipunder grant no. DGE-1247321 and NIH NIGMS 5T32GM008764. A.C.S.N.P. thanks L. G. de la Torre, University ofCampinas (UNICAMP), and is grateful forfinancial supportfrom São Paulo Research Foundation (FAPESP) (process no.2018/05278-0)

The development of new therapies for surgical adhesions has proven to be difficult as there is no consistently effective way to assess treatment efficacy in clinical trials without performing a second surgery, which can result in additional adhesions. We have developed lipid microbubble formulations...

The development of new therapies for surgical adhesions has proven to be difficult as there is no consistently effective way to assess treatment efficacy in clinical trials without performing a second surgery, which can result in additional adhesions. We have developed lipid microbubble formulations that use a short peptide sequence, CREKA, to target fibrin, the molecule that forms nascent adhesions. These targeted polymerized shell microbubbles (PSMs) are designed to allow ultrasound imaging of early adhesions for diagnostic purposes and for evaluating the success of potential treatments in clinical trials while acting as a possible treatment. In this study, we show that CREKA-targeted microbubbles preferentially bind fibrin over fibrinogen and are stable for long periods of time (similar to 48 h), that these bound microbubbles can be visualized by ultrasound, and that neither these lipid-based bubbles nor their diagnostic-ultrasound-induced vibrations damage mesothelial cells in vitro. Moreover, these bubbles show the potential to identify adhesionlike fibrin formations and may hold promise in blocking or breaking up fibrin formations in vivo

FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP

2018/05278-0

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