Validation of the sulfo-phospho-vanillin (SPV) method for the determination of lipid content in oleaginous microorganisms
A. Anschau, C. S. Caruso, R. C. Kuhn, T. T. Franco
ARTIGO
Inglês
A colorimetric sulfo-phospho-vanillin (SPV) method was used to validate a high throughput method for total lipids analysis in fresh and lyophilized oleaginous microorganisms. This method uses a small amount of biological material, does not require a lot of sample manipulation, can be automated, is...
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A colorimetric sulfo-phospho-vanillin (SPV) method was used to validate a high throughput method for total lipids analysis in fresh and lyophilized oleaginous microorganisms. This method uses a small amount of biological material, does not require a lot of sample manipulation, can be automated, is reproducible and easy to implement. A 96-well microplate SPV assay allows one to determine quickly total lipids in lyophilized cells of oleaginous yeast and microalgae. The new assay method possesses many advantages compared to the others described in the literature: requires a small amount sample, less time (around 1 h) and less labor and does not require organic reagents like chloroform in the reaction
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CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
Aberto
Validation of the sulfo-phospho-vanillin (SPV) method for the determination of lipid content in oleaginous microorganisms
A. Anschau, C. S. Caruso, R. C. Kuhn, T. T. Franco
Validation of the sulfo-phospho-vanillin (SPV) method for the determination of lipid content in oleaginous microorganisms
A. Anschau, C. S. Caruso, R. C. Kuhn, T. T. Franco
Fontes
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Brazilian journal of chemical engineering Vol. 34, no. 1 (Jan./Mar., 2017), p. 19-27 |