The cyanobacteria have great potential for aquaculture industries, bioproducts, bioenergy and bioremediation, as in the reduction of ammonia, phosphorus and organic compounds. The manipulation of the medium and the modes of operation of the cultures are necessary to induce the production of biomass...

The cyanobacteria have great potential for aquaculture industries, bioproducts, bioenergy and bioremediation, as in the reduction of ammonia, phosphorus and organic compounds. The manipulation of the medium and the modes of operation of the cultures are necessary to induce the production of biomass and bioproducts, such as lipids. The aim of the study was to evaluate the different nitrogen sources and their depletion in heterotrophic cultures of the cyanobacterium Phormidium autumnale aimed at obtaining high yields lipid. The heterotrophic cultures were carried out in bubble column bioreactor employing cassava starch as organic carbon source. In the first stage, were studied different nitrogen source under the C/N (carbon/nitrogen) ratios in the culture medium of 20, 40 and 60. In the second stage, selected to nitrogen source with the greatest potential for the production of lipids and held its depletion searching for the induction of a cellular stress. The fatty acids profile in the end of cultures was used in the estimation of biodiesel quality. In the first stage, the cultures employing sodium nitrate under CN ratio of 60 resulting in lipid content of 13.22% and lipid productivity of 7.62 mg/L.h. From the depletion of sodium nitrate, second stage, obtained a lipid productivity and lipid content of 10.43 mg/L.h and 25.07%, respectively. The fatty acid profile showed, in the presence of nitrogen, saturated fatty acid fraction and monounsaturated of 76.72% and 23.88%, respectively. The depletion of sodium nitrate induced a change in the lipid profile, directing the concentration of saturated fatty acids of 98.97%. The biodiesel properties obtained meet the standards set by standardization of the quality of biodiesel

FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP

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