The purpose of this paper is to present a comparative study on the characteristics of knitted fabrics used in the manufacturing of apparel, which are produced from organic cotton, lyocell and soybean protein fiber (SPF). It is important for both the environment and society that textile industry continues to adopt more ecofriendly materials and furthermore, pushes to increase awareness regarding these material choices available to the consumer and the corresponding impacts of consumers’ decisions. The use of sustainable fibers may be a starting point for changing the industrial paradigm of the textile industry. Design/methodology/approach – The research presented herein analyzes the potential use of three raw materials used in the development of knitted fabrics: organic cotton, lyocell and SPF. The experimental trials, based on norms, determined the weight, pilling, rupture pressure resistance, absorption by capillarity, dimensional alteration and elasticity. The significance of the experimental results was verified through the analysis of variance, with a confidence interval of 95 percent (p¼ 0.05) and the determination of the optimal regulation of the machine was made through an analysis of the response surface. The results indicate that each of the studied materials are suitable for textile application; however, the fabrics manufactured from soybean yarn, compared to those manufactured from organic cotton or lyocell, have a higher potential to meet the needs of the costumer. Social implications – The discussion regarding sustainability is far reaching on the ways it interacts with human life. As such, the latent need for meeting this new demand presents a unique opportunity for the development of new processes and products. In the case of the textile industry, initiatives are gradually being adopted that make the processes used by the supply chain less damaging to the environment. Clothing and fashion are highly visible elements of society, so consequently, the textile industry serves as an excellent candidate for promoting a sustainable and eco-friendly mindset. Originality/value – The incorporation of sustainable fibers can serve as a starting point for change to the industrial paradigm existing within the textile industry. To this point, this study intended to analyze the potential implementation of three raw materials – organic cotton, lyocell and SPF – in the development of knitted fabrics. The results indicated that these materials are adequate for textile applications Ver menos