HA/TCP scaffolds coated by PLA and gelatin : preliminary in vitro evaluation
Leonardo Ribeiro Rodrigues, Cecília Amélia de Carvalho Zavaglia, Christiane Bertachini Lombello
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Agradecimentos: The authors are thankful for the financial support from Fundação Universidade Federal do ABC (UFABC) and INCT-BioFabris. Carlos Leite for his support in the TEM analysis. Professor Marcos Akira d'Ávila and Professor Sonia Maria Malmonge for provision of laboratory reagents,...
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Agradecimentos: The authors are thankful for the financial support from Fundação Universidade Federal do ABC (UFABC) and INCT-BioFabris. Carlos Leite for his support in the TEM analysis. Professor Marcos Akira d'Ávila and Professor Sonia Maria Malmonge for provision of laboratory reagents, respectively type B gelatin and PLA. Professor Oswaldo Luiz Alves for allowing me to use the SkyScan Micro-CT
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The importance of this work is development of two methods to try to improve the bone tissue regeneration. The surface of scaffold was modified in order to favor cell interaction, through adhesion and proliferation. PLA and gelatin were used. PLA has shown in literature good results in bone tissue...
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The importance of this work is development of two methods to try to improve the bone tissue regeneration. The surface of scaffold was modified in order to favor cell interaction, through adhesion and proliferation. PLA and gelatin were used. PLA has shown in literature good results in bone tissue engineering. The gelatin is used as coating in cell culture plates to improve cell attachment for a variety of cell types, including osteoblast. Hydroxyapatite (HA) and tricalcium phosphate (TCP) were used due to its known properties in tissue engineering. It was used the polymer foam replication technique to produce the scaffolds. For characterization were used: scanning electron microscopy (SEM), optical microscopy (OP), stereoscopy, transmission electron microscopy (TEM), X-ray microtomography (Micro-CT), X-ray diffraction (XRD) and X-ray fluorescence (XRF). The scaffolds showed morphology with adequate porosity for tissue engineering and the in vitro test showed evidence of not being cytotoxic
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HA/TCP scaffolds coated by PLA and gelatin : preliminary in vitro evaluation
Leonardo Ribeiro Rodrigues, Cecília Amélia de Carvalho Zavaglia, Christiane Bertachini Lombello
HA/TCP scaffolds coated by PLA and gelatin : preliminary in vitro evaluation
Leonardo Ribeiro Rodrigues, Cecília Amélia de Carvalho Zavaglia, Christiane Bertachini Lombello
Fontes
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Key Engineering Materials Vol. 631 (Nov., 2014), p. 289-294 |