Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/97931
Type: Artigo
Title: Toluidine blue binding capacity of heterochromatin and euchromatin of Triatoma infestans Klug
Author: Mello, M.L.S.
Abstract: The localization of the absorption peaks of toluidine blue-stained heterochromatin (H(o) and H(s)) and euchromatin (E(o) and E(s)) of the Malpighian tubules of the blood-sucking hemipteran, Triatoma infestans Klug was studied cytophotometrically (H(o) and E(o): hetero- and echromatin of nuclei with one single chromocenter; H(s) and E(s): hetero- and echromatin of nuclei with several chromocenters). Shifts of the absorption peaks to longer wave-lengths were found for the euchromatin fractions and H(s) after RNAse treatment. The differences in the localization of absorption peaks after RNAse digestion when comparing the various chromatin types with one another are assumed to be due to differences in the number and proximity of DNA phosphates unattached to proteins and hence available to dye binding. The localization of the absorption peak of H(o) at a relatively short wavelength (λ=570 nm) as compared with those for H(s), E(o) and E(s) is attributed to a special state of chromatin condensation and practical absence of RNA in H(o). Also a certain removal of histones (probably, H1) by the acetic ethanol 'fixation' is speculated to play a part in the absorption curve properties of H(o) subjected to TB staining.
The localization of the absorption peaks of toluidine blue-stained heterochromatin (H(o) and H(s)) and euchromatin (E(o) and E(s)) of the Malpighian tubules of the blood-sucking hemipteran, Triatoma infestans Klug was studied cytophotometrically (H(o) and E(o): hetero- and echromatin of nuclei with one single chromocenter
H(s) and E(s): hetero- and echromatin of nuclei with several chromocenters). Shifts of the absorption peaks to longer wave-lengths were found for the euchromatin fractions and H(s) after RNAse treatment. The differences in the localization of absorption peaks after RNAse digestion when comparing the various chromatin types with one another are assumed to be due to differences in the number and proximity of DNA phosphates unattached to proteins and hence available to dye binding. The localization of the absorption peak of H(o) at a relatively short wavelength (λ=570 nm) as compared with those for H(s), E(o) and E(s) is attributed to a special state of chromatin condensation and practical absence of RNA in H(o). Also a certain removal of histones (probably, H1) by the acetic ethanol 'fixation' is speculated to play a part in the absorption curve properties of H(o) subjected to TB staining.
Subject: Heterocromatina
Eucromatina
Azul de toluidina
Espectro de absorção
Túbulos de Malpighi
Triatoma infestans
Country: Alemanha
Editor: Springer
Citation: Histochemistry. , v. 69, n. 2, p. 181 - 187, 1980.
Rights: fechado
Identifier DOI: 10.1007/BF00533135
Address: https://link-springer-com.ez88.periodicos.capes.gov.br/article/10.1007/BF00533135
Date Issue: 1980
Appears in Collections:IB - Artigos e Outros Documentos

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