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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.subject.otherPANCREATIC BETA-CELLSen
dc.subject.otherGENE-EXPRESSIONen
dc.subject.otherRECEPTOR SUBSTRATE-1en
dc.subject.otherMETABOLIC-REGULATIONen
dc.subject.otherAUTOCRINE REGULATIONen
dc.subject.otherENDOCRINE PANCREASen
dc.subject.otherSIGNALING PATHWAYen
dc.subject.otherCA2+ HOMEOSTASISen
dc.subject.otherALPHA EXPRESSIONen
dc.subject.otherDEFICIENT RATSen
dc.typeArtigo de periódicopt_BR
dc.type.categoryoriginal articlept_BR
dc.type.versionpublishedVersionpt_BR
dc.titleAugmentation of insulin secretion by leucine supplementation in malnourished rats: possible involvement of the phosphatidylinositol 3-phosphate kinase/mammalian target protein of rapamycin pathwaypt_BR
dc.contributor.authorFILIPUTTI, Elianept_BR
dc.contributor.authorRAFACHO, Alexpt_BR
dc.contributor.authorARAUJO, Eliana P.pt_BR
dc.contributor.authorSILVEIRA, Leonardo R.pt_BR
dc.contributor.authorTREVISAN, Amonpt_BR
dc.contributor.authorBATISTA, Thiago M.pt_BR
dc.contributor.authorCURI, Ruipt_BR
dc.contributor.authorVELLOSO, Licio A.pt_BR
dc.contributor.authorQUESADA, Ivanpt_BR
dc.contributor.authorBOSCHERO, Antonio C.pt_BR
dc.contributor.authorCARNEIRO, Everardo M.pt_BR
unicamp.authorFILIPUTTI, Eliane:Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Anatomia, Biologia Celular, Fisiologia e Biofísicapt_BR
unicamp.authorRAFACHO, Alex:Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Anatomia, Biologia Celular, Fisiologia e Biofísicapt_BR
unicamp.authorARAUJO, Eliana P.:Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Anatomia, Biologia Celular, Fisiologia e Biofísicapt_BR
unicamp.authorTREVISAN, Amon:Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Anatomia, Biologia Celular, Fisiologia e Biofísicapt_BR
unicamp.authorBATISTA, Thiago M.:Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Anatomia, Biologia Celular, Fisiologia e Biofísicapt_BR
unicamp.authorVELLOSO, Licio A.:Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Anatomia, Biologia Celular, Fisiologia e Biofísicapt_BR
unicamp.authorBOSCHERO, Antonio C.:Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Anatomia, Biologia Celular, Fisiologia e Biofísicapt_BR
unicamp.authorCARNEIRO, Everardo M.:Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Anatomia, Biologia Celular, Fisiologia e Biofísicapt_BR
unicamp.author.externalSILVEIRA, Leonardo R.pt
unicamp.author.externalQUESADA, Ivanpt
unicamp.author.externalCURI, Ruipt
dc.subject.wosEndocrinology & Metabolismpt_BR
dc.description.abstractA regimen of low-protein diet induces a reduction of pancreatic islet function that is associated with development of metabolic disorders including diabetes and obesity afterward. In the present study, the influence of leucine supplementation on metabolic parameters, insulin secretion to glucose and to amino acids, as well as the levels of proteins that participate in the phosphatidylinositol 3-phosphate kinase (PI3K) pathway was investigated in malnourished rats. Four groups were fed with different diets for 12 weeks: a normal protein diet (17%) without (NP) or with leucine supplementation (NPL) or a low (6%)-protein diet without (LP) or with leucine supplementation (LPL). Leucine was given in the drinking water during the last 4 weeks. As indicated by the intraperitoneal glucose tolerance test, LPL rats exhibited increased glucose tolerance as compared with NPL group. Both NPL and LPL rats had higher circulating insulin levels than controls. The LPL rats also showed increased insulin secretion by pancreatic islets in response to glucose or arginine compared with those observed in islets from LP animals. Glucose oxidation was significantly reduced in NPL, LP, and LPL isolated islets as compared with NP; but no alteration was observed for leucine and glutamate oxidation among the 4 groups. Western blotting analysis demonstrated increased PI3K and mammalian target protein of rapamycin protein contents in LPL compared with LP islets. A significant increase in insulin-induced insulin receptor substrate I associated PI3K activation was also observed in LPL compared with LP islets. These findings indicate that leucine supplementation can augment islet function in malnourished rats and that activation of the PI3K/maminalian target protein of rapamycin pathway may play a role in this process. (C) 2010 Elsevier Inc. All rights reserved.en
dc.relation.ispartofMetabolism-clinical and Experimentalpt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherW B SAUNDERS CO-ELSEVIER INCpt_BR
dc.date.issued2010pt_BR
dc.identifier.citationMETABOLISM-CLINICAL AND EXPERIMENTAL, v.59, n.5, p.635-644, 2010pt_BR
dc.language.isoengpt_BR
dc.description.volume59pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage635pt_BR
dc.description.lastpage644pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright W B SAUNDERS CO-ELSEVIER INCpt_BR
dc.sourceWOSpt_BR
unicamp.cruespUSPpt_BR
dc.identifier.issn0026-0495pt_BR
dc.identifier.doi10.1016/j.metabol.2009.09.007pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1016/j.metabol.2009.09.007pt_BR
dc.identifier.urlhttp://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000277103700004&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecordpt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.date.available2013-07-26T17:58:48Z
dc.date.available2016-07-01T14:37:05Z-
dc.date.accessioned2013-07-26T17:58:48Z
dc.date.accessioned2016-07-01T14:37:05Z-
dc.description.provenanceMade available in DSpace on 2013-07-26T17:58:48Z (GMT). No. of bitstreams: 2 art_FILIPUTTI_Augmentation_of_insulin_secretion_by_leucine_supplementation_2010.pdf.txt: 49205 bytes, checksum: cab3eccf07e7dfa8dd2dbb66e9ea9c86 (MD5) art_FILIPUTTI_Augmentation_of_insulin_secretion_by_leucine_supplementation_2010.pdf: 820027 bytes, checksum: 451bf434c9379ba71e30cbb4f2779fb2 (MD5) Previous issue date: 2010en
dc.description.provenanceMade available in DSpace on 2016-07-01T14:37:05Z (GMT). No. of bitstreams: 2 art_FILIPUTTI_Augmentation_of_insulin_secretion_by_leucine_supplementation_2010.pdf.txt: 49205 bytes, checksum: cab3eccf07e7dfa8dd2dbb66e9ea9c86 (MD5) art_FILIPUTTI_Augmentation_of_insulin_secretion_by_leucine_supplementation_2010.pdf: 820027 bytes, checksum: 451bf434c9379ba71e30cbb4f2779fb2 (MD5) Previous issue date: 2010en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/929
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/929-
dc.contributor.departmentAnatomia
dc.contributor.unidadeIBpt
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