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Type: Artigo de periódico
Title: In Vitro And In Vivo Anti-malarial Activity Of Limonoids Isolated From The Residual Seed Biomass From Carapa Guianensis (andiroba) Oil Production
Author: Pereira T.B.
Rocha E Silva L.F.
Amorim R.C.
Melo M.R.
Zacardi De Souza R.C.
Eberlin M.N.
Lima E.S.
Vasconcellos M.C.
Pohlit A.M.
Abstract: Background: Carapa guianensis is a cultivable tree used by traditional health practitioners in the Amazon region to treat several diseases and particularly symptoms related to malaria. Abundant residual pressed seed material (RPSM) results as a by-product of carapa or andiroba oil production. The objective of this study was to evaluate the in vitro and in vivo anti-malarial activity and cytotoxicity of limonoids isolated from C. guaianensis RPSM.Methods. 6-acetoxyepoxyazadiradione (1), andirobin (2), 6-acetoxygedunin (3) and 7-deacetoxy-7-oxogedunin (4) (all isolated from RPSM using extraction and chromatography techniques) and 6-hydroxy-deacetylgedunin (5) (prepared from 3) were evaluated using the micro test on the multi-drug-resistant Plasmodium falciparum K1 strain. The efficacy of limonoids 3 and 4 was then evaluated orally and subcutaneously in BALB/c mice infected with chloroquine-sensitive Plasmodium berghei NK65 strain in the 4-day suppressive test.Results: In vitro, limonoids 1-5 exhibited median inhibition concentrations (IC50) of 20.7-5.0 μM, respectively. In general, these limonoids were not toxic to normal cells (MRC-5 human fibroblasts). In vivo, 3 was more active than 4. At oral doses of 50 and 100 mg/kg/day, 3 suppressed parasitaemia versus untreated controls by 40 and 66%, respectively, evidencing a clear dose-response.Conclusion: 6-acetoxygedunin is an abundant natural product present in C. guianensis residual seed materials that exhibits significant in vivo anti-malarial properties.
Editor: BioMed Central Ltd.
Citation: Malaria Journal. Biomed Central Ltd., v. 13, n. 1, p. - , 2014.
Rights: aberto
Identifier DOI: 10.1186/1475-2875-13-317
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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