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Type: Artigo de periódico
Title: Optimization of enantioselective resolution of racemic ibuprofen by native lipase from Aspergillus niger
Author: Carvalho, PD
Contesini, FJ
Bizaco, R
Calafatti, SA
Macedo, GA
Abstract: Resolution of (R,S)-ibuprofen (2-(4-isobutylphenyl)propionic acid) enantiomers by esterification reaction with 1-propanol in different organic solvents was studied using native Aspergillus niger lipase. The main variables controlling the process (enzyme concentration and 1-propanol:ibuprofen molar ratio) have been optimized using response surface methodology based on a five-level, two-variable central composite rotatable design, in which the selected objective function was enantioselectivity. This enzyme preparation showed preferentially catalyzes the esterification of R(-)-ibuprofen, and under optimum conditions (7% w/v of enzyme and molar ratio of 2.41:1) the enantiomeric excess of active S(+)-ibuprofen and total conversion values were 79.1 and 48.0%, respectively, and the E-value was 32, after 168 h of reaction in isooctane.
Subject: (R,S)-ibuprofen
Aspergillus niger
Country: Alemanha
Editor: Springer Heidelberg
Citation: Journal Of Industrial Microbiology & Biotechnology. Springer Heidelberg, v. 33, n. 8, n. 713, n. 718, 2006.
Rights: fechado
Identifier DOI: 10.1007/s10295-006-0138-8
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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