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Type: Artigo
Title: Inhibition of eosinophil chemotaxis by chronic blockade of nitric oxide biosynthesis
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Author: Ferreira, H.H.A.
Lima, C.S.P.
Medeiros, M.V.
Sannomiya, P.
Flores, C.A.
Antunes, E.
De Nucci, G.
Abstract: The effect of chronic N-omega-nitro-L-arginine methyl ester (L-NAME) treatment on the in vivo eosinophil migration induced by bradykinin, platelet-activating factor (PAF), lipopolysaccharide and carrageenin has been investigated in the rat using the pleurisy model, The in vitro (microchemotaxis chamber) eosinophil migration induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP), PAF and zymosan-activated serum was also evaluated in the rat. The eosinophils were obtained from the peritoneal cavity of male Wistar rats and isolated on a discontinuous metrizamide gradient. Chronic inhibition of nitric oxide biosynthesis was achieved by adding L-NAME to the drinking water to give an intake of approximately 75 mu mol/rat/day for 4 weeks. Rats treated chronically with L-NAME developed a significant level of hypertension (163 +/- 4.8 mmHg; P < 0.01) compared with animals which received either the same dose of the inactive enantiomer D-NAME (124 +/- 3.2 mmHg) or tap water alone (119 +/- 1.6 mmHg). The intrapleural injection of bradykinin (50 mu g), PAF (1 mu g), lipopolysaccharide (0.25 mu g) and carrageenin (125 mu g) into untreated rats in vivo induced a significant level of eosinophil migration by 24 h post-injection. This migration was markedly reduced in L-NAME-treated rats. Eosinophils obtained from untreated rats showed a significant level of migration in vitro in response to fMLP (5 x 10(-8) M), PAF (10(-8) M) and zymosan-activated serum (27 mu l). In contrast, the migration induced by these chemotactic agents was markedly reduced in cells isolated from animals treated chronically with L-NAME. L-Arginine (5.5 mM), but not D-arginine (5.5 mM), restored the ability of eosinophils from L-NAME-treated animals to migrate in response to fMLP. Our results indicate that nitric oxide plays a major role in the in vivo and ex vivo migration of eosinophils
Subject: Óxido nítrico
Country: Países Baixos
Editor: Elsevier
Citation: European Journal Of Pharmacology. Elsevier Science Bv, v. 310, n. 41700, n. 201, n. 207, 1996.
Rights: fechado
Identifier DOI: 10.1016/0014-2999(96)00379-2
Date Issue: 1996
Appears in Collections:FCM - Artigos e Outros Documentos

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