Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/79757
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampGerlach, Raquel Fernandapt_BR
dc.contributor.authorunicampde Souza, Ana Paulapt_BR
dc.contributor.authorunicampCury, Jaime Aparecidopt_BR
dc.contributor.authorunicampLine, Sergio Roberto Perespt_BR
dc.typeArtigopt_BR
dc.titleEffect of lead, cadmium and zinc on the activity of enamel matrix proteinases in vitropt_BR
dc.contributor.authorGerlach, R. F.pt_BR
dc.contributor.authorde Souza, A. P.pt_BR
dc.contributor.authorCury, J. A.pt_BR
dc.contributor.authorLine, S. R. P.pt_BR
unicamp.authorUNICAMP, Dent Sch Piracicaba, Fac Odontol Piracicaba, Dept Morphol, BR-13414018 Piracicaba, SP, Brazil UNICAMP, Dent Sch Piracicaba, Dept Physiol Sci, BR-13414018 Piracicaba, SP, Brazilpt_BR
dc.subjectRatospt_BR
dc.subjectMetais pesadospt_BR
dc.subjectDentinapt_BR
dc.subjectMatriz extracelularpt_BR
dc.subject.otherlanguageRatspt_BR
dc.subject.otherlanguageHeavy metalspt_BR
dc.subject.otherlanguageDentinpt_BR
dc.subject.otherlanguageExtracellular matrixpt_BR
dc.subject.wosSerine Proteinase-1pt_BR
dc.subject.wosHuman-teethpt_BR
dc.subject.wosCalciumpt_BR
dc.subject.wosToothpt_BR
dc.subject.wosAmelogeninpt_BR
dc.subject.wosMechanismspt_BR
dc.subject.wosActivationpt_BR
dc.subject.wosFluoridept_BR
dc.subject.wosExposurept_BR
dc.subject.wosMetalloproteinase-20pt_BR
dc.description.abstractEnvironmental contamination with heavy metals leads to their uptake by the body. Dental tissues are known to harbor metals in concentrations related to the exposure at the time of dentin or enamel formation. Several enzymes involved in the metabolism of extracellular matrix components have been shown to be inhibited by excess metal ions. Enamel matrix proteinases seem to play a central role in the maturation of dental enamel, and inhibition of these enzymes by metals could interfere with amelogenesis. We have investigated the effect of lead, cadmium and zinc on the activity of enamel matrix proteinases using a colorimetric assay, casein and gelatin zymography, and an assay of enamel protein degradation in vitro. All three metals inhibited the proteolysis, as shown by the three methods employed, in concentrations as low as 110 mu M. This study showed that contaminant metals can inhibit proteinolysis when a crude enamel extract containing enamel proteases is tested in vitro. Therefore, the presence of heavy metals in enamel matrix could interfere with amelogenesis due to alteration in normal proteolysispt
dc.relation.ispartofEuropean journal of oral sciencespt_BR
dc.relation.ispartofabbreviationEur j oral scipt_BR
dc.publisher.cityChichesterpt_BR
dc.publisher.countryInglaterrapt_BR
dc.publisherWileypt_BR
dc.date.issued2000pt_BR
dc.date.monthofcirculationAug.pt_BR
dc.identifier.citationEuropean Journal Of Oral Sciences. Munksgaard Int Publ Ltd, v. 108, n. 4, n. 327, n. 334, 2000.pt_BR
dc.language.isoengpt_BR
dc.description.volume108pt_BR
dc.description.issuenumber4pt_BR
dc.description.firstpage327pt_BR
dc.description.lastpage334pt_BR
dc.rightsabertopt_BR
dc.sourceWeb of Sciencept_BR
dc.sourceWOSpt_br
dc.identifier.issn0909-8836pt_BR
dc.identifier.eissn1600-0722pt_BR
dc.identifier.wosidWOS:000088477000010pt_BR
dc.identifier.doi10.1034/j.1600-0722.2000.108004327.xpt_BR
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1034/j.1600-0722.2000.108004327.xpt_BR
dc.description.sponsorshipFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorship1FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsordocumentnumber1997/12210-5; 1997/1365-5pt_BR
dc.date.available2014-12-02T16:25:20Z
dc.date.available2015-11-26T17:21:54Z-
dc.date.accessioned2014-12-02T16:25:20Z
dc.date.accessioned2015-11-26T17:21:54Z-
dc.description.provenanceMade available in DSpace on 2014-12-02T16:25:20Z (GMT). No. of bitstreams: 1 WOS000088477000010.pdf: 203458 bytes, checksum: 91f4d2bc65c27fae591daab494b11e24 (MD5) Previous issue date: 2000en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:21:54Z (GMT). No. of bitstreams: 2 WOS000088477000010.pdf: 203458 bytes, checksum: 91f4d2bc65c27fae591daab494b11e24 (MD5) WOS000088477000010.pdf.txt: 36765 bytes, checksum: 24bd05d9b2c1e80f5d952eab1203e252 (MD5) Previous issue date: 2000en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79757pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/79757
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79757-
dc.contributor.departmentDepartamento de Morfologiapt_BR
dc.contributor.departmentDepartamento de Ciências Fisiológicaspt_BR
dc.contributor.departmentDepartamento de Morfologiapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.subject.keywordZymographypt_BR
dc.identifier.source88477000010-
dc.creator.orcid0000-0002-9769-4859pt_BR
dc.creator.orcid0000-0003-1046-5605pt_BR
dc.creator.orcid0000-0002-6574-9464pt_BR
dc.type.formArtigo-
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