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Type: Artigo de periódico
Title: Decreased insulin secretion in islets from rats fed a low protein diet is associated with a reduced PKA alpha expression
Author: Ferreira, F
Barbosa, HCL
Stoppiglia, LF
Delghingaro-Augusto, V
Pereira, EA
Boschero, AC
Carneiro, EM
Abstract: A low protein diet has been shown to affect the amount and activity of several enzymes and to decrease insulin secretion by islets isolated from rats fed such a diet. To understand the mechanisms involved in this phenomenon, we investigated the effects of forskolin, a stimulator of adenylyl cyclase, on insulin secretion by pancreatic islets from rats fed a normal (17%; NP) or low (6%; LP) protein diet for 8 wk. Isolated islets were incubated for 1 h in Krebs-bicarbonate solution containing 8.3 mmol glucose/L, with or without 10 mumol forskolin/L. The forskolin-induced insulin secretion was higher in islets from NP rats than in those from LP rats (P < 0.05). Western blotting revealed that the amount of the alpha catalytic subunit of protein kinase A (PKAalpha) was 35% lower in islets from LP rats than in islets from NP rats (P < 0.05). Moreover, PKAalpha mRNA expression was reduced by 30% in islets from LP rats (P < 0.05). Our results indicated a possible relationship between a low protein diet and a reduction in PKAalpha expression. These alterations in PKAalpha may be responsible in part for the decreased insulin secretion by islets from rats fed a low protein diet.
Subject: low protein diet
insulin secretion
protein kinase alpha
gene expression
Country: EUA
Editor: Amer Inst Nutrition
Citation: Journal Of Nutrition. Amer Inst Nutrition, v. 134, n. 1, n. 63, n. 67, 2004.
Rights: fechado
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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