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Type: Artigo de periódico
Title: Walker 256 tumor MHC class I expression during the shift from A variant to the immunogenic AR variant
Author: Schanoski, AS
Cavalcanti, TC
Barbosa, C
Campos, L
Viera-Matos, AN
Rettori, O
Guimaraes, F
Abstract: Novel tumor cell variants can be obtained by serially passaging tumor cells in different media and/or environments. Serial intraperitoneal (ip) passages of the Walker 256 tumor A variant was followed for studying the generation of its regressive AR variant. MHC class I molecule expression was assessed since variations in this molecule would explain changes in tumor cell immunogenicity and therefore, the shift from progressive A variant to the regressive AR variant. Within 25 ip passages all serial repetitions shifted from A to AR variant, which was characterized by a significant increase in red blood cell (R13Q osmotic fragility with marked spleen hypertrophy in the host. In one serial repetition AR tumor cells were rejected tip passage number 36) and immunity against the AR and A variants was conferred. Flow cytometry analysis showed a significant increase in the number MHC class I positive cells in AR variant (n = 15, 14.21 +/- 1.32) compared with A variant (n = 10, 9.10 +/- 1.22). These data provide evidence that the generation of the AR variant could result from factors present in the ip environment leading to an increase in the number of Walker 256 MHC class I positive tumor cells, probably due to immune selection of MHC class I negative tumor cells. (C) 2004 Published by Elsevier Ireland Ltd.
Subject: AMC class I expression
ascitic tumor
tumor cell variants
Walker 256 tumor
Country: Irlanda
Editor: Elsevier Sci Ireland Ltd
Citation: Cancer Letters. Elsevier Sci Ireland Ltd, v. 211, n. 1, n. 119, n. 127, 2004.
Rights: fechado
Identifier DOI: 10.1016/j.canlet.2004.01.023
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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