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Type: Artigo de periódico
Title: Serobiotypes and virulence genes of Escherichia coli strains isolated from diarrheic and healthy rabbits in Brazil
Author: Penteado, AS
Ugrinovich, LA
Blanco, J
Blanco, M
Blanco, JE
Mora, A
Andrade, JRC
Correa, SS
de Castro, AFP
Abstract: A total of 171 Escherichia coli isolates from diarrheic and healthy rabbit, in the Sao Paulo Slate (Brazil) were serobiotyped and investigated by PCR for the presence of virulence genes. Among the 90 (.50.6%) isolates which possessed the eae gene, 74 were from diarrheic animals and all but one encoded intimin beta. Sixty five (72.2%) of the eae+ isolates had insertion of the locus of enterocyte effacement locus in the pheU locus, 11 (12.2%) in the selC and 14 (15.6%) did not insert in either of these loci. All isolates were negative for genes of the E. coli enterotoxins, Stx1, Stx2, CNF1, CNF2 and EHEC hemolysin. The 0132:H2 serotype was dominant, being present in 63 isolates (70%) of the 90 eae+ isolates, and 57 of the 63 isolates of this serotype belonged to biotype 30. PCR detected the gene for AF/R2 fimbriae in 75 (83.3%) of the 90 eae+ isolates. Adherence to HeLa cells was best detected following 6 h incubation and a positive fluorescence actin staining (FAS) test was given by 52 isolates. These data show that isolates of E. coli associated with diarrhea in rabbits in Brazil possess the genotype and phenotype typically associated with rabbit enteropathogenic E. coli (EPEC). We conclude that EPEC that possess the eae gene are a common cause of diarrhea in Brazilian rabbit farms and that the pathogenic eae+ AF/R2+ isolates of 0132:H2:B30 serobiotype are especially predominant. (C) 2002 Elsevier Science B.V. All rights reserved.
Subject: Escherichia coli
attaching and effacing
eae gene
Country: Holanda
Editor: Elsevier Science Bv
Citation: Veterinary Microbiology. Elsevier Science Bv, v. 89, n. 1, n. 41, n. 51, 2002.
Rights: fechado
Identifier DOI: 10.1016/S0378-1135(02)00148-7
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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