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|Type:||Artigo de periódico|
|Title:||The Con-A-peroxidase method for tissue localization of glucosyl and mannosyl groups applied to mouse hepatocytes and chicken erythrocytes|
|Abstract:||A variation of the Concanavalin A (Con-A)-peroxidase labelling method originally described by Kiernan [Localization of alpha-D-glucosyl and alpha-D-mannosyl groups of mucosubstances with Concanavalin A and horseradish peroxidase. Histochemistry 1975;44:39-45] was applied to unsectioned cell preparations, with an emphasis on the nuclear localization of glycoproteins. Mouse liver imprints and chicken blood smears fixed in acetic acid-ethanol solution were studied. Modifications of the. method included using increased Con-A concentration, and a range of pH values for the Con-A solutions. The strongest Con-A labelling of both erythrocytes and hepatocytes was obtained after incubation with Con-A at pH 6.5 and with Con-A concentrations at least two-fold greater than those used for tissue sections. These conditions may alter the Con-A conformation, enabling the lectin molecule to enter the cell nucleus and bind to nuclear glycoproteins, thus allowing their localization and quantification. (C) 2006 Elsevier GmbH. All rights reserved.|
|Editor:||Elsevier Gmbh, Urban & Fischer Verlag|
|Citation:||Acta Histochemica. Elsevier Gmbh, Urban & Fischer Verlag, v. 108, n. 6, n. 475, n. 479, 2006.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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