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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleSimultaneous analysis of first-line anti-tuberculosis drugs in tablets by UV spectrophotometry compared to capillary zone electrophoresispt_BR
dc.contributor.authorMarcellos, LFpt_BR
dc.contributor.authorFaria, AFpt_BR
dc.contributor.authorde Souza, MVNpt_BR
dc.contributor.authorAlmeida, MRpt_BR
dc.contributor.authorSabin, GPpt_BR
dc.contributor.authorPoppi, RJpt_BR
dc.contributor.authorde Oliveira, MALpt_BR
unicamp.authorMarcellos, Luiza F. Faria, Adriana F. de Oliveira, Marcone A. L. Univ Fed Juiz de Fora, Dept Chem ICE, BR-36036330 Juiz De Fora, MG, Brazilpt_BR
unicamp.authorde Souza, Marcus V. N. Fundacao Oswaldo Cruz, Inst Technol Pharmaceut Farmanguinhos, BR-21041250 Rio De Janeiro, Brazilpt_BR
unicamp.authorAlmeida, Mariana R. Sabin, Guilherme P. Poppi, Ronei J. Univ Estadual Campinas, Inst Chem, BR-13084971 Campinas, SP, Brazilpt_BR
dc.subjectUV spectrophotometrypt_BR
dc.subjectCapillary electrophoresispt_BR
dc.subject.wosFixed-dose Combinationpt_BR
dc.subject.wosPharmaceutical Formulationspt_BR
dc.subject.wosPulmonary Tuberculosispt_BR
dc.description.abstractThe development and optimization of a novel UV spectrophotometric methodology was proposed for simultaneous analysis of ethambutol (ETB), isoniazid (ISO), rifampicin (RIF) and pyrazinamide (PYR), using multivariate calibration based on the partial least squares method (PLS). The methodology was successfully applied for analysis of four-drug fixed dose combination (4-FDC) tablets used for tuberculosis treatment. A 3(4) Box-Behnken design, with triplicate in central point, was used for sample preparation in the calibration step. In the present case, nine latent variables were chosen for the model development that presented the smallest RMSECV and explain 98.76% of data variance in Y block (concentrations of ETB ISO, RIF and PYR) and 99.93% of data variance in X block (spectral data). PLS models for ETB, ISO, RIF and PYR presented RMSEP and R-2 values of 0.23 mg L-1 and 0.971; 0.14 mg L-1 and 0.731; 0.11 mg L-1 and 0.990 and 0.57 mg L-1 and 0.972, respectively. A validation step was performed based on the comparison between the UV spectrophotometric proposed methodology and capillary zone electrophoresis (CZE) in 4-FDC real samples and no significant difference was found between two methodologies at 95% of confidence
dc.relation.ispartofCentral European Journal Of Chemistrypt_BR
dc.relation.ispartofabbreviationCent. Eur. J. Chempt_BR
dc.identifier.citationCentral European Journal Of Chemistry. Versita, v. 10, n. 6, n. 1808, n. 1816, 2012.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsordocumentnumberCNPq [CNPq - 300593/2008-2]pt
dc.description.sponsordocumentnumberFundacao de Amparo a Pesquisa do Estado de Minas Gerais of Brazil [FAPEMIG - CEX - APQ-02420-11, CEX - PPM-00205-11]pt
dc.description.provenanceMade available in DSpace on 2014-07-30T19:57:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
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