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Type: Artigo de periódico
Title: Use of a sequential strategy of experimental design to optimize the inulinase production in a batch bioreactor
Author: Treichel, H
Mazutti, MA
Maugeri, F
Rodrigues, MI
Abstract: A potential application of inulinase in the food industry is the production of fructoligosaccharides (FOS) by the transfructosilation of sucrose. The FOSs present many interesting functional properties besides their ability to increase the shelf-life and flavor of many products. The use of an industrial medium represents a good alternative to producing inulinase at low cost, since the activity may improve, or at least remain the same, as that obtained using a synthetic medium. This work was an optimization study of the inulinase production by Kluyveromyces marxianus NRRL Y-7571 using industrial pre-treated culture medium in a bioreactor employing a sequential strategy of experimental design. Initially, a Plackett-Burman (Screening Design) design was used, where the studied variables were molasses, corn steep liquor, yeast extract concentration, and agitation and aeration rates. After the analysis of the effects, a central composite rotational design (CCRD) was carried out. The optimized condition for the inulinase production was: 250 g/l of molasses, 80 g/l of corn steep liquor, 6 g/l of yeast extract, 300 rpm of agitation and 1.5 vvm aeration rate, which resulted in an enzymatic activity of 1,317 +/- A 65 U/ml.
Subject: Inulinase
Kluyveromyces marxianus NRRL Y-7571
Batch bioreactor
Agro-industrial residues
Country: Alemanha
Editor: Springer Heidelberg
Citation: Journal Of Industrial Microbiology & Biotechnology. Springer Heidelberg, v. 36, n. 7, n. 895, n. 900, 2009.
Rights: fechado
Identifier DOI: 10.1007/s10295-009-0567-2
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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