Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/73121
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampBarbosa, Raquel de Melopt_BR
dc.contributor.authorunicampFranz-Montan, Michellept_BR
dc.contributor.authorunicampGrillo, Renatopt_BR
dc.contributor.authorunicampPaula, Eneida dept_BR
dc.typeArtigopt_BR
dc.titleValidation of an HPLC method for the determination of dibucaine encapsulated in solid lipid nanoparticles and nanostructured lipid carrierspt_BR
dc.contributor.authorBarbosa, R. D.pt_BR
dc.contributor.authorKlassen, A.pt_BR
dc.contributor.authorMarcato, P. D.pt_BR
dc.contributor.authorFranz-Montan, M.pt_BR
dc.contributor.authorGrillo, R.pt_BR
dc.contributor.authorFraceto, L. F.pt_BR
dc.contributor.authorde Paula, E.pt_BR
unicamp.author.emaildepaula@unicamp.brpt_BR
unicamp.authorBarbosa, Raquel de M. Franz-Montan, Michelle Grillo, Renato de Paula, Eneida State Univ Campinas UNICAMP, Dept Biochem, BR-13083862 Campinas, SP, Brazilpt_BR
unicamp.authorKlassen, Aline Univ Sao Paulo, Inst Chem, BR-05513970 Sao Paulo, Brazilpt_BR
unicamp.authorMarcato, Priscyla D. Univ Sao Paulo, Fac Pharmaceut Sci Riberao Preto, BR-05513970 Sao Paulo, Brazilpt_BR
unicamp.authorGrillo, Renato Fraceto, Leonardo F. Sao Paulo State Univ UNESP, Dept Environm Engn, BR-18087180 Sorocaba, SP, Brazilpt_BR
dc.subjectDibucaínapt_BR
dc.subject.otherlanguageDibucainept_BR
dc.subject.wosChromatography-mass-spectrometrypt_BR
dc.subject.wosLocal-anestheticspt_BR
dc.subject.wosTopical Deliverypt_BR
dc.subject.wosLiquid-chromatographypt_BR
dc.subject.wosSkin Permeationpt_BR
dc.subject.wosDermal Deliverypt_BR
dc.subject.wosSlnpt_BR
dc.subject.wosDrugspt_BR
dc.subject.wosNanocarrierspt_BR
dc.subject.wosFormulationspt_BR
dc.description.abstractDibucaine (DBC), used mainly in topical formulations, is one of the most potent long-acting local anesthetics, but is also one of the more toxic. Recently, solid lipid nanoparticles and nanostructured lipid carriers have been attracting attention as promising drug delivery carriers. This study develops and validates an analytical HPLC method for quantifying the local anesthetic DBC associated to lipidic structures. Average sizes, polydispersion, surface charge and DBC encapsulation efficiency were analyzed. The DBC quantification was performed by using C18-reversed-phase column, a mobile phase with acetonitrile: triethylamine phosphate buffer and UV detection. The results show that the analytical methodology is accurate, reproducible and robust; the method was linear in the concentration range 1.5-30 mu g/mL with a high correlation coefficient (r = 0.999). The nanoparticles presented mean diameters around 200 nm and high encapsulation efficiency for DBC (over 70% for SLN and NLC samples). This methodology can be useful for quantifying DBC in different nanostructured carrierspt
dc.relation.ispartofLatin american journal of pharmacypt_BR
dc.relation.ispartofabbreviationLat. am. j. pharm.pt_BR
dc.publisher.cityLa Platapt_BR
dc.publisher.countryArgentinapt_BR
dc.publisherColegio de Farmaceuticos de la Provincia de Buenos Airespt_BR
dc.date.issued2013pt_BR
dc.identifier.citationLatin American Journal Of Pharmacy. Colegio Farmaceuticos Provincia De Buenos Aires, v. 32, n. 9, n. 1362, n. 1369, 2013.pt_BR
dc.language.isoengpt_BR
dc.description.volume32pt_BR
dc.description.issuenumber9pt_BR
dc.description.firstpage1362pt_BR
dc.description.lastpage1369pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.sourceWOSpt_br
unicamp.cruespUNESP USPpt_BR
dc.identifier.issn0326-2383pt_BR
dc.identifier.wosidWOS:000331252200013pt_BR
dc.identifier.urlhttp://www.latamjpharm.org/previous_issue.php?vol=32&num=9pt_BR
dc.description.sponsorshipFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorshipCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorship1FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorship1CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsordocumentnumberFAPESP [06/00121-9]pt
dc.description.sponsordocumentnumber2006/00121-9pt_BR
dc.date.available2014-07-30T19:07:39Z
dc.date.available2015-11-26T16:55:29Z-
dc.date.accessioned2014-07-30T19:07:39Z
dc.date.accessioned2015-11-26T16:55:29Z-
dc.description.provenanceMade available in DSpace on 2014-07-30T19:07:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2013. Added 1 bitstream(s) on 2019-03-14T14:32:27Z : No. of bitstreams: 1 000331252200013.pdf: 39222 bytes, checksum: ccc2c97cb3107d00213e09c2e6bd257b (MD5)en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:55:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/73121
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/73121-
dc.contributor.departmentInformação não localizadapt_BR
dc.contributor.departmentInformação não localizadapt_BR
dc.contributor.departmentInformação não localizadapt_BR
dc.contributor.departmentDepartamento de Bioquímica e Biologia Tecidualpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordHPLCpt_BR
dc.subject.keywordLipid carrierspt_BR
dc.subject.keywordMethod validationpt_BR
dc.identifier.source331252200013-
dc.creator.orcidInformação não localizadapt_BR
dc.creator.orcid0000-0003-0760-1389pt_BR
dc.creator.orcidInformação não localizadapt_BR
dc.creator.orcid0000-0003-4504-5723pt_BR
dc.type.formArtigo-
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