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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleUsefulness of concanavalin-A non-binding fraction of Strongyloides venezuelensis larvae to detect IgG and IgA in human strongyloidiasispt_BR
dc.contributor.authorGonzaga, HTpt_BR
dc.contributor.authorRibeiro, VDpt_BR
dc.contributor.authorCunha, JPpt_BR
dc.contributor.authorUeta, MTpt_BR
dc.contributor.authorCosta-Cruz, JMpt_BR
unicamp.author.emailcostacruz@ufu.brpt_BR
unicamp.authorGonzaga, Henrique Tomaz Ribeiro, Vanessa da Silva Cunha-Junior, Jair Pereira Costa-Cruz, Julia Maria Univ Fed Uberlandia, Inst Ciencias Biomed, Dept Imunol Microbiol & Parasitol, BR-38400902 Uberlandia, MG, Brazilpt_BR
unicamp.authorUeta, Marlene Tiduko Univ Estadual Campinas, Inst Biol, Dept Parasitol, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjectStrongyloidiasispt_BR
dc.subjectStrongyloides stercoralispt_BR
dc.subjectGlycoproteinspt_BR
dc.subjectSerodiagnosispt_BR
dc.subject.wosLinked-immunosorbent-assaypt_BR
dc.subject.wosHuman Neurocysticercosispt_BR
dc.subject.wosStercoralis Infectionpt_BR
dc.subject.wosIntestinal Nematodept_BR
dc.subject.wosAntigenic Fractionspt_BR
dc.subject.wosTaenia-saginatapt_BR
dc.subject.wosDiagnosispt_BR
dc.subject.wosResponsespt_BR
dc.subject.wosImmunodiagnosispt_BR
dc.subject.wosReactivitypt_BR
dc.description.abstractGlycosylated components from Strongyloides have an important role in parasite establishment and host recognition of these substances. Considering the sugar-binding capacity of lectins, such as concanavalin-A (Con-A), IgG and IgA detection in serum samples from strongyloidiasis patients was tested using different antigenic preparations. The total saline extract (SE) of Strongyloides venezuelensis filariform larvae was fractionated in Con-A column to obtain Con-A unbound (Con-A UF) and Con-A bound (Con-A BF) fractions. Sensitivity (Se), specificity (Sp), area under the ROC curve (AUC), likelihood ratio (LR), and correlation coefficients were calculated. Con-A UF showed the highest diagnostic parameters for IgG detection (Se 95.0%, Sp 92.5%, AUC 0.99, LR 12.7) and high correlation (r = 0.700) with SE. Con-A fractions did not clearly demonstrate any usefulness for IgA detection. In conclusion, the results obtained demonstrate that Con-A UF is an important source of specific peptides efficient to detect IgG in strongyloidiasis immunodiagnosis. (C) 2011 Elsevier Inc. All rights reserved.pt
dc.relation.ispartofDiagnostic Microbiology And Infectious Diseasept_BR
dc.relation.ispartofabbreviationDiagn. Microbiol. Infect. Dis.pt_BR
dc.publisher.cityNew Yorkpt_BR
dc.publisher.countryEUApt_BR
dc.publisherElsevier Science Incpt_BR
dc.date.issued2011pt_BR
dc.date.monthofcirculationMAYpt_BR
dc.identifier.citationDiagnostic Microbiology And Infectious Disease. Elsevier Science Inc, v. 70, n. 1, n. 78, n. 84, 2011.pt_BR
dc.language.isoenpt_BR
dc.description.volume70pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage78pt_BR
dc.description.lastpage84pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0732-8893pt_BR
dc.identifier.wosidWOS:000290290500012pt_BR
dc.identifier.doi10.1016/j.diagmicrobio.2011.01.016pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)pt_BR
dc.description.sponsorshipFederal University of Uberlandia (UFU), Brazilpt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.date.available2014-07-30T19:07:29Z
dc.date.available2015-11-26T16:28:01Z-
dc.date.accessioned2014-07-30T19:07:29Z
dc.date.accessioned2015-11-26T16:28:01Z-
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dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/73039
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/73039-
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