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Type: Artigo de periódico
Title: Usefulness of concanavalin-A non-binding fraction of Strongyloides venezuelensis larvae to detect IgG and IgA in human strongyloidiasis
Author: Gonzaga, HT
Ribeiro, VD
Cunha, JP
Ueta, MT
Costa-Cruz, JM
Abstract: Glycosylated components from Strongyloides have an important role in parasite establishment and host recognition of these substances. Considering the sugar-binding capacity of lectins, such as concanavalin-A (Con-A), IgG and IgA detection in serum samples from strongyloidiasis patients was tested using different antigenic preparations. The total saline extract (SE) of Strongyloides venezuelensis filariform larvae was fractionated in Con-A column to obtain Con-A unbound (Con-A UF) and Con-A bound (Con-A BF) fractions. Sensitivity (Se), specificity (Sp), area under the ROC curve (AUC), likelihood ratio (LR), and correlation coefficients were calculated. Con-A UF showed the highest diagnostic parameters for IgG detection (Se 95.0%, Sp 92.5%, AUC 0.99, LR 12.7) and high correlation (r = 0.700) with SE. Con-A fractions did not clearly demonstrate any usefulness for IgA detection. In conclusion, the results obtained demonstrate that Con-A UF is an important source of specific peptides efficient to detect IgG in strongyloidiasis immunodiagnosis. (C) 2011 Elsevier Inc. All rights reserved.
Subject: Strongyloidiasis
Strongyloides stercoralis
Country: EUA
Editor: Elsevier Science Inc
Citation: Diagnostic Microbiology And Infectious Disease. Elsevier Science Inc, v. 70, n. 1, n. 78, n. 84, 2011.
Rights: fechado
Identifier DOI: 10.1016/j.diagmicrobio.2011.01.016
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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