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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleWestern diet modulates insulin signaling, c-jun N-terminal kinase activity, and insulin receptor substrate-1(ser307) phosphorylation in a tissue-specific fashionpt_BR
dc.contributor.authorPrada, POpt_BR
dc.contributor.authorZecchin, HGpt_BR
dc.contributor.authorGasparetti, ALpt_BR
dc.contributor.authorTorsoni, MApt_BR
dc.contributor.authorUeno, Mpt_BR
dc.contributor.authorHirata, AEpt_BR
dc.contributor.authordo Amaral, MECpt_BR
dc.contributor.authorHoer, NFpt_BR
dc.contributor.authorBoschero, ACpt_BR
dc.contributor.authorSaad, MJApt_BR
unicamp.author.emailmsaad@fcm.unicamp.brpt_BR
unicamp.authorUniv Estadual Campinas, Dept Clin Med, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subject.wosObese Zucker Ratspt_BR
dc.subject.wosPhosphatidylinositol 3-kinasept_BR
dc.subject.wosTranscription Factorspt_BR
dc.subject.wosInduced Anorexiapt_BR
dc.subject.wosArcuate Nucleuspt_BR
dc.subject.wosAdipose-tissuept_BR
dc.subject.wosBody-weightpt_BR
dc.subject.wosResistancept_BR
dc.subject.wosMicept_BR
dc.subject.wosMusclept_BR
dc.description.abstractThe mechanisms by which diet-induced obesity is associated with insulin resistance are not well established, and no study has until now integrated, in a temporal manner, functional insulin action data with insulin signaling in key insulin-sensitive tissues, including the hypothalamus. In this study, we evaluated the regulation of insulin sensitivity by hyperinsulinemic-euglycemic clamp procedures and insulin signaling, c-jun N-terminal kinase (JNK) activation and insulin receptor substrate (IRS)-1(ser307) phosphorylation in liver, muscle, adipose tissue, and hypothalamus, by immunoprecipitation and immunoblotting, in rats fed on a Western diet (WD) or control diet for 10 or 30 d. WD increased visceral adiposity, serum triacylglycerol, and insulin levels and reduced whole-body glucose use. After 10 d of WD (WD10) there was a decrease in IRS-1/phosphatidylinositol 3-kinase/protein kinase B pathway in hypothalamus and muscle, associated with an attenuation of the anorexigenic effect of insulin in the former and reduced glucose transport in the latter. In WD10, there was an increased glucose transport in adipose tissue in parallel to increased insulin signaling in this tissue. After 30 d of WD, insulin was less effective in suppressing hepatic glucose production, and this was associated with a decrease in insulin signaling in the liver. JNK activity and IRS-1(ser307) phosphorylation were higher in insulin-resistant tissues. In summary, the insulin resistance induced by WD is tissue specific and installs first in hypothalamus and muscle and later in liver, accompanied by activation of JNK and IRS-1(ser307) phosphorylation. The impairment of the insulin signaling in these tissues, but not in adipose tissue, may lead to increased adiposity and insulin resistance in the WD rats.pt
dc.description.noteo TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.pt
dc.relation.ispartofEndocrinologypt_BR
dc.relation.ispartofabbreviationEndocrinologypt_BR
dc.publisher.cityChevy Chasept_BR
dc.publisher.countryEUApt_BR
dc.publisherEndocrine Socpt_BR
dc.date.issued2005pt_BR
dc.date.monthofcirculationMARpt_BR
dc.identifier.citationEndocrinology. Endocrine Soc, v. 146, n. 3, n. 1576, n. 1587, 2005.pt_BR
dc.language.isoenpt_BR
dc.description.volume146pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage1576pt_BR
dc.description.lastpage1587pt_BR
dc.rightsembargopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0013-7227pt_BR
dc.identifier.wosidWOS:000227035400070pt_BR
dc.identifier.doi10.1210/en.2004-0767pt_BR
dc.date.available2014-07-30T19:02:48Z
dc.date.available2015-11-26T16:55:24Z-
dc.date.accessioned2014-07-30T19:02:48Z
dc.date.accessioned2015-11-26T16:55:24Z-
dc.description.provenanceMade available in DSpace on 2014-07-30T19:02:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2005en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:55:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2005en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/72837
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/72837-
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