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Type: Artigo de periódico
Title: The role of transient receptor potential A 1 (TRPA1) in the development and maintenance of carrageenan-induced hyperalgesia
Author: Bonet, IJM
Fischer, L
Parada, CA
Tambeli, CH
Abstract: Transient receptor potential ankyrin 1 (TRPA1) is a nonselective cation channel important in setting nociceptive threshold. It is expressed in nociceptive C-fibers and in non-neuronal cells involved in pro-inflammatory mediators' release. We asked whether TRPA1 contributes to carrageenan-induced hyperalgesia in rats, and if so, whether this contribution is mediated by mechanisms involved in inflammation such as cytokine release and neutrophil migration and/or by a direct sensitization of the primary afferent nociceptors. Pharmacological blockade of local TRPA1 by its selective antagonist HC 030031 prevented and reversed carrageenan-induced hyperalgesia, which was detected either by a mechanical or chemical (low dose of capsaicin) stimulus. However, it did not affect either carrageenan-induced cytokines expression or neutrophil migration. The neuronal TRPA1 gene silencing induced by intrathecal pretreatment with antisense oligodoexynucleotide completely prevented carrageenan-induced hyperalgesia over 24 h and significantly reduced TRPA1 expression in the dorsal root ganglia cells (L5-6), which was not affected by carrageenan treatment. We conclude that TRPA1 plays an important role in the development and maintenance of carrageenan-induced inflammatory hyperalgesia by directly contributing to nociceptor excitability. (C) 2012 Elsevier Ltd. All rights reserved.
Subject: TRPA1
Nociceptor sensitization
Neutrophil migration
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Citation: Neuropharmacology. Pergamon-elsevier Science Ltd, v. 65, n. 206, n. 212, 2013.
Rights: fechado
Identifier DOI: 10.1016/j.neuropharm.2012.09.020
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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