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Type: Artigo de periódico
Title: Operation of a fixed-bed bioreactor in batch and fed-batch modes for production of inulinase by solid-state fermentation
Author: Astolfi, V
Joris, J
Verlindo, R
Oliveira, JV
Maugeri, F
Mazutti, MA
de Oliveira, D
Treichel, H
Abstract: This work is focused on the inulinase production by solid-state fermentation (SSF) in a fixed-bed reactor (34 cm diameter and 50 cm height) with working capacity of 2-kg of dry substrate operated in batch and fed-batch modes. It was investigated different strategies for feeding the inlet air in the bioreactor (saturated and unsaturated air) as alternative to remove the metabolic heat generated during the microbial growth by evaporative cooling. The kinetic evaluation of the process carried out in batch mode using unsaturated air showed that the evaporative cooling decreasing the mean temperature of the solid-bed, although the enzyme production was lower than that obtained using saturated air. Results showed that maximum enzyme activity (586 +/- 63 Ugds(-1)) was obtained in the fed-batch mode using saturated air after 24h of fermentation. The enzymatic extract obtained by fed-batch mode was characterized and presented optimum temperature and pH in the range of 52-57 degrees C and 4.8-5.2, respectively. For a temperature range from 40 to 70 degrees C the enzyme presented decimal reduction time, D-value, ranging from 5748 to 47h, respectively. For a pH range from 3.5 to 5.5 the enzyme showed good stability, presenting D-values higher than 2622 h. In terms of Michaelis-Mentem parameters were demonstrated that the crude inulinase activity presented higher affinity for substrate sucrose compared to inulin. (C) 2011 Elsevier B.V. All rights reserved.
Subject: Fed-batch fermentation
Agroindustrial residues
Kluyveromyces marxianus NRRL Y-7571
Partial characterization
Solid-state fermentation
Country: Suíça
Editor: Elsevier Science Sa
Citation: Biochemical Engineering Journal. Elsevier Science Sa, v. 58-59, n. 39, n. 49, 2011.
Rights: fechado
Identifier DOI: 10.1016/j.bej.2011.08.012
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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