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Type: Artigo de periódico
Title: Identification of Cross-Linked Peptides by High-Resolution Precursor Ion Scan
Author: Iglesias, AH
Santos, LFA
Gozzo, FC
Abstract: Chemical cross-linking coupled to mass spectrometry analysis has become a realistic alternative to the study of proteins structure and interactions, especially when these systems are not amenable to high-resolution techniques such as protein crystallography or nuclear magnetic resonance. One of the main bottlenecks of this approach relies on the detection of cross-linked peptides, as they are usually present in substoichiometric amounts in complex samples. It was shown that one of the main fragmentation pathways of disuccinimidyl suberate (DSS) cross-linked peptides yields diagnostic ions, whose structure is composed of a rearranged lysine side chain and the spacer arm of the linker. In this report, we demonstrate the feasibility of detecting these modified peptides based on a precursor ion scan in a quadrupole time-of-flight (Q-TOF) instrument. It was shown that the fragmentation of nonmodified tryptic peptides hardly generates ions with the same nominal mass of the diagnostic ions, making the precursor ion scan very specific to N-hydroxysuccinimide (NHS)-based cross-linkers. Moreover, the experimental setup is the same as in the case of a regular cross-linking experiment, not demanding any additional experimental steps that would increase sample handling. The results obtained with protein samples allowed us to propose an algorithm that could be implemented in a software to process data from cross-linking experiments in an automated and high-throughput way.
Country: EUA
Editor: Amer Chemical Soc
Citation: Analytical Chemistry. Amer Chemical Soc, v. 82, n. 3, n. 909, n. 916, 2010.
Rights: fechado
Identifier DOI: 10.1021/ac902051q
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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