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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleHydrophobic fractions from Strongyloides venezuelensis for use in the human immunodiagnosis of strongyloidiasispt_BR
dc.contributor.authorFeliciano, NDpt_BR
dc.contributor.authorGonzaga, HTpt_BR
dc.contributor.authorGoncalves-Pires, MDFpt_BR
dc.contributor.authorGoncalves, ALRpt_BR
dc.contributor.authorRodrigues, RMpt_BR
dc.contributor.authorUeta, MTpt_BR
dc.contributor.authorCosta-Cruz, JMpt_BR
unicamp.author.emailcostacruz@ufu.brpt_BR
unicamp.authorFeliciano, Nagilla Daliane Gonzaga, Henrique Tomaz Fatima Goncalves-Pires, Maria do Rosario Ribeiro Goncalves, Ana Lucia Rodrigues, Rosangela Maria Costa-Cruz, Julia Maria Univ Fed Uberlandia, Dept Microbiol Immunol & Parasitol, Inst Ciencias Biomed, Lab Diagnost Parasitoses, BR-38400902 Uberlandia, MG, Brazilpt_BR
unicamp.authorUeta, Marlene Tiduko Univ Estadual Campinas, Inst Biol, Dept Parasitol, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjectHydrophobic fractionspt_BR
dc.subjectImmunodiagnosispt_BR
dc.subjectStrongyloides venezuelensispt_BR
dc.subjectStrongyloidiasispt_BR
dc.subject.wosLinked-immunosorbent-assaypt_BR
dc.subject.wosStercoralis Infectionpt_BR
dc.subject.wosDiagnosispt_BR
dc.subject.wosAntibodiespt_BR
dc.subject.wosProteinspt_BR
dc.subject.wosAntigenpt_BR
dc.subject.wosRattipt_BR
dc.subject.wosImmunofluorescencept_BR
dc.description.abstractThe objective of the present research was to evaluate detergent and aqueous phases of total saline (TS) and alkaline extracts of Strongyloides venezuelensis for human strongyloidiasis immunodiagnosis. Total extracts and detergent and aqueous antigenic fractions were separated using Triton X-114 and were examined by enzyme-linked immunosorbent assay (ELISA) and immunoblotting (IB) tests to detect immunoglobulin G (IgG). Serum samples were obtained from 120 individuals: 40 strongyloidiasis patients (group I), 40 patients with other parasitic diseases (group II), and 40 apparently healthy individuals (group III). Each extract provided a different profile of antigenic components as recognized by IgG in TB. The detergent fraction of the TS extract demonstrated the highest sensitivity and specificity for ELISA and IB. The results indicated that the detergent saline fraction, purified from S. venezuelensis, furnished the most valid results for the strongyloidiasis immunodiagnosis and could be employed as an alternative antigen and as a useful source of specific polypeptides. (C) 2010 Elsevier Inc. All rights reserved.pt
dc.relation.ispartofDiagnostic Microbiology And Infectious Diseasept_BR
dc.relation.ispartofabbreviationDiagn. Microbiol. Infect. Dis.pt_BR
dc.publisher.cityNew Yorkpt_BR
dc.publisher.countryEUApt_BR
dc.publisherElsevier Science Incpt_BR
dc.date.issued2010pt_BR
dc.date.monthofcirculationJUNpt_BR
dc.identifier.citationDiagnostic Microbiology And Infectious Disease. Elsevier Science Inc, v. 67, n. 2, n. 153, n. 161, 2010.pt_BR
dc.language.isoenpt_BR
dc.description.volume67pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage153pt_BR
dc.description.lastpage161pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0732-8893pt_BR
dc.identifier.wosidWOS:000278194700006pt_BR
dc.identifier.doi10.1016/j.diagmicrobio.2010.01.012pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFederal University of Uberlandia, Brazilpt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.date.available2014-11-14T05:18:12Z
dc.date.available2015-11-26T16:04:39Z-
dc.date.accessioned2014-11-14T05:18:12Z
dc.date.accessioned2015-11-26T16:04:39Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:04:39Z (GMT). No. of bitstreams: 2 WOS000278194700006.pdf: 675728 bytes, checksum: 444449d8e7d48d98b126e3d33532bf77 (MD5) WOS000278194700006.pdf.txt: 34880 bytes, checksum: 08a9aa2a8c59dcc3243404df6a5252c7 (MD5) Previous issue date: 2010en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69023pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/69023
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69023-
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