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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleOestrogen imprinting causes nuclear changes in epithelial cells and overall inhibition of gene transcription and protein synthesis in rat ventral prostatept_BR
dc.contributor.authorAugusto, TMpt_BR
dc.contributor.authorBruni-Cardoso, Apt_BR
dc.contributor.authorDamas-Souza, DMpt_BR
dc.contributor.authorZambuzzi, WFpt_BR
dc.contributor.authorKuhne, Fpt_BR
dc.contributor.authorLourenco, LBpt_BR
dc.contributor.authorFerreira, CVpt_BR
dc.contributor.authorCarvalho, HFpt_BR
unicamp.author.emailhern@unicamp.brpt_BR
unicamp.authorAugusto, T. M. Bruni-Cardoso, A. Damas-Souza, D. M. Kuehne, F. Lourenco, L. B. Carvalho, H. F. State Univ Campinas UNICAMP, Dept Anat Cell Biol Physiol & Biophys, Inst Biol, BR-13083863 Campinas, SP, Brazilpt_BR
unicamp.authorZambuzzi, W. F. Ferreira, C. V. State Univ Campinas UNICAMP, Dept Biochem, Inst Biol, BR-13083863 Campinas, SP, Brazilpt_BR
dc.subject4EBPpt_BR
dc.subjectandrogen receptorpt_BR
dc.subjectoestrogen imprintingpt_BR
dc.subjectmTORpt_BR
dc.subjectnucleoluspt_BR
dc.subjectprostatept_BR
dc.subjectprotein synthesispt_BR
dc.subject.wosRibosomal-rna Genespt_BR
dc.subject.wosNeonatal Estrogenpt_BR
dc.subject.wosPostnatal-developmentpt_BR
dc.subject.wosAndrogen Receptorpt_BR
dc.subject.wosGlandpt_BR
dc.subject.wosMethylationpt_BR
dc.subject.wosExposurept_BR
dc.subject.wosGrowthpt_BR
dc.subject.wosExpressionpt_BR
dc.subject.wosEstradiolpt_BR
dc.description.abstractOestrogen exposure during the early post-natal period affects male growth, physiology, and susceptibility to disease in adult life. The prostate gland is susceptible to this oestrogen imprinting, showing a reduced expression of the androgen receptor and inability to respond to androgen stimulus. In this context, we decided to study key signalling regulators of ventral prostate (VP) functioning after early postnatal exposure to high-dose oestrogen. Our results showed a decrease of mTOR phosphorylation and its direct downstream target 4EBP. It is known that mTOR-induced signalling is a pivotal pathway of cell metabolism, which is able to control gene transcription and protein synthesis. We then decided to investigate other indicators of a reduced metabolism in the oestrogenized prostate, and found that the luminal epithelial cells were shorter, less polarized and had smaller nuclei containing more compacted chromatin, suggesting that a general mechanism of regulating gene expression and protein synthesis could be installed in the epithelium of the oestrogenized VP. To evaluate this idea, we analysed nucleolar morphology, and measured the amount of ribosomes and the level of methylation of the 45S ribosomal RNA promoter region. These data indicated that the nucleolus was dismantled and that the methylation at the 45S promoter was increased (similar to five-fold). Taken together, the results support the idea that the oestrogenized prostate maintains a very low transcriptional level and protein turnover by affecting canonical signalling pathways and promoting nuclear and nucleolar changes.pt
dc.relation.ispartofInternational Journal Of Andrologypt_BR
dc.relation.ispartofabbreviationInt. J. Androl.pt_BR
dc.publisher.cityMaldenpt_BR
dc.publisher.countryEUApt_BR
dc.publisherWiley-blackwellpt_BR
dc.date.issued2010pt_BR
dc.date.monthofcirculationOCTpt_BR
dc.identifier.citationInternational Journal Of Andrology. Wiley-blackwell, v. 33, n. 5, n. 675, n. 685, 2010.pt_BR
dc.language.isoenpt_BR
dc.description.volume33pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage675pt_BR
dc.description.lastpage685pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlpt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0105-6263pt_BR
dc.identifier.wosidWOS:000281555900002pt_BR
dc.identifier.doi10.1111/j.1365-2605.2009.01008.xpt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFAPESP [05/02601-5, 08/53003-9]pt
dc.date.available2014-11-14T01:17:17Z
dc.date.available2015-11-26T16:03:31Z-
dc.date.accessioned2014-11-14T01:17:17Z
dc.date.accessioned2015-11-26T16:03:31Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:03:31Z (GMT). No. of bitstreams: 2 WOS000281555900002.pdf: 505306 bytes, checksum: d1d3e8db55325c2a64b1f14e78d76441 (MD5) WOS000281555900002.pdf.txt: 45352 bytes, checksum: 9e2ef715fe0f8b60dc02064cfd2ed8f6 (MD5) Previous issue date: 2010en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/67555pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/67555
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/67555-
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