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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleEnzymatic recovery and purification of polyhydroxybutyrate produced by Ralstonia eutrophapt_BR
dc.contributor.authorKapritchkoff, FMpt_BR
dc.contributor.authorViotti, APpt_BR
dc.contributor.authorAlli, RCPpt_BR
dc.contributor.authorZuccolo, Mpt_BR
dc.contributor.authorPradella, JGCpt_BR
dc.contributor.authorMaiorano, AEpt_BR
dc.contributor.authorMiranda, EApt_BR
dc.contributor.authorBonomi, Apt_BR
unicamp.author.emailferkapri@yahoo.compt_BR
unicamp.authorIPT, Agrupamento Biotecnol DQ, BR-05508901 Sao Paulo, Brazil Univ Estadual Campinas, Fac Engn Quim, Dept Proc Biotecnol, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjectpolyhydroxyalkanoatespt_BR
dc.subjectdownstream processingpt_BR
dc.subjectpurificationpt_BR
dc.subjectenzymatic processpt_BR
dc.subjectcell rupturept_BR
dc.subjectenzymespt_BR
dc.subject.wosAlcaligenes-eutrophuspt_BR
dc.subject.wosPoly(hydroxyalkanoates)pt_BR
dc.subject.wosPseudomonadspt_BR
dc.description.abstractPolyhydroxybutyrate (PHB) is the most studied among a wide variety of polyhydroxyalkanoates, bacterial biodegradable polymers known as potential substitutes for conventional plastics. This work aimed at evaluating the use of enzymes to recover and purify the PHB produced by Ralstonia eutropha DSM545. Screening experiments allowed the selection of trypsin, bromelain and lysozyme among six enzymes, based on their efficiency in lysing cells of a non-PHB producing R. eutropha strain. Then, process conditions for high efficiency in PRB purification from the DSM545 cells were searched for the enzymes previously selected. The best result was achieved with 2.0% of bromelain (enzyme mass per biomass), equivalent to 14.1 U ml(-1), at 50 degrees C and pH 9.0, resulting in 88.8% PHB purity. Aiming at improving the process efficiency and reducing the enzyme cost, experiments were carried out with pancreatin, leading to 90.0% polymer purity and an enzyme cost three times lower than the one obtained with bromelain. The molecular mass analysis of PHB showed no polymer degradation. Therefore, this work demonstrates the potential of using enzymes in order to recover and purify PHB and bacterial biopolymers in general. (c) 2005 Elsevier B.V. All rights reserved.pt
dc.relation.ispartofJournal Of Biotechnologypt_BR
dc.relation.ispartofabbreviationJ. Biotechnol.pt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryHolandapt_BR
dc.publisherElsevier Science Bvpt_BR
dc.date.issued2006pt_BR
dc.date.monthofcirculationAPR 20pt_BR
dc.identifier.citationJournal Of Biotechnology. Elsevier Science Bv, v. 122, n. 4, n. 453, n. 462, 2006.pt_BR
dc.language.isoenpt_BR
dc.description.volume122pt_BR
dc.description.issuenumber4pt_BR
dc.description.firstpage453pt_BR
dc.description.lastpage462pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0168-1656pt_BR
dc.identifier.wosidWOS:000236823800006pt_BR
dc.identifier.doi10.1016/j.jbiotec.2005.09.009pt_BR
dc.date.available2014-11-17T22:36:29Z
dc.date.available2015-11-26T16:47:15Z-
dc.date.accessioned2014-11-17T22:36:29Z
dc.date.accessioned2015-11-26T16:47:15Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:47:15Z (GMT). No. of bitstreams: 2 WOS000236823800006.pdf: 192010 bytes, checksum: d8070442bd1abbe169713c06f7b3a069 (MD5) WOS000236823800006.pdf.txt: 37159 bytes, checksum: 0491e396dde9e6871a27c93ef9b6f609 (MD5) Previous issue date: 2006en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64942pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/64942
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64942-
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