Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/642
Type: Artigo de periódico
Title: Cytotoxicity and genotoxicity of bovine pericardium preserved in glycerol
Author: RODAS, Andrea C. D.
MAIZATO, Marina J. S.
LEIRNER, Adolfo A.
PITOMBO, Ronaldo N. M.
POLAKIEWICZ, Bronislaw
BEPPU, Marisa M.
HIGA, Olga Z.
Abstract: Bovine pericardium is a widely utilized biomaterial. Usually, after harvesting, it is advantageous that the pericardium be immersed in glycerol to improve its shelf life. This can induce some degree of toxicity in the material. The studies were performed in compliance with the rules of ISO 10993 and OECD 487, in the biological evaluation of medical devices. The material was prepared without previous washing. After sterilization by gamma radiation the pericardium was immersed in RPMI 1640 culture medium to fulfill the extraction condition. The same extract was employed in the cytotoxic and genotoxic tests. The procedures were carried out with Chinese hamster ovary cell line and to determine the cytotoxicity, a colorimetric method with the tetrazolium compound MTS was used. For the genotoxicity, following the in vitro micronucleus assay, the test was developed with and without metabolic activation. The Cytotoxicity Index was graphically estimated at the extract concentration of 78%. In the genotoxicity test, the average value of cell proliferation index was found to be 1.62 +/- 0.02 with S9 metabolic activator and 1.91 +/- 0.01 without S9 metabolic activator. Both values are similar to the negative control value in the micronucleus assay. We observed that although the pericardium preserved in glycerol shows a certain level of cytotoxicity, it does not show any genotoxicity.
Subject: cytotoxicity
genotoxicity
glycerol
pericardium
Country: Estados Unidos
Editor: WILEY-BLACKWELL PUBLISHING, INC
Citation: ARTIFICIAL ORGANS, v.32, n.4, p.272-276, 2008
Rights: fechado
Identifier DOI: 10.1111/j.1525-1594.2008.00542.x
Address: http://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000254412900004&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
http://dx.doi.org/10.1111/j.1525-1594.2008.00542.x
Date Issue: 2008
Appears in Collections:FEQ - Artigos e Outros Documentos

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