Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/64085
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampKoo, Hyunpt_BR
dc.contributor.authorunicampRosalen, Pedro Luizpt_BR
dc.contributor.authorunicampCury, Jaime Aparecidopt_BR
dc.contributor.authorunicampPark, Yong Kunpt_BR
dc.typeArtigopt_BR
dc.titleEffects of Apis mellifera propolis on the activities of streptococcal glucosyltransferases in solution and adsorbed onto saliva-coated hydroxyapatitept_BR
dc.contributor.authorKoo, H.pt_BR
dc.contributor.authorSmith, A. M. V.pt_BR
dc.contributor.authorBowen, W. H.pt_BR
dc.contributor.authorRosalen, P. L.pt_BR
dc.contributor.authorCury, J. A.pt_BR
dc.contributor.authorPark, Y. K.pt_BR
dc.subjectPrópolispt_BR
dc.subjectHidroxiapatitapt_BR
dc.subject.otherlanguagePropolispt_BR
dc.subject.otherlanguageHydroxyapatitept_BR
dc.description.abstractPropolis, a resinous hive product collected by Apis mellifera bees, has been used for thousands of years in folk medicine. Ethanolic extracts of propolis (EEP) have been shown to inhibit the activity of a mixture of crude glucosyltransferase (Gtf) enzymes in solution. These enzymes synthesize glucans from sucrose, which are important for the formation of pathogenic dental plaque. In the present study, the effects of propolis from two different regions of Brazil on the activity of separate, purified Gtf enzymes in solution and on the surface of saliva-coated hydroxyapatite (sHA) beads were evaluated. The EEP from Minas Gerais (MG; Southeastern Brazil) and Rio Grande do Sul (RS; Southern Brazil) were tested for their ability to inhibit the enzymes GtfB (synthesis of insoluble glucan), GtfC (insoluble/soluble glucan) and GtfD (soluble glucan). The effects of propolis on Gtf from Streptococcus sanguis (soluble glucan synthesis) was also explored. The EEP from both regions effectively inhibited the activity of all Gtfs in solution (75-95%) and on the surface of sHA beads (45-95%) at concentrations between 0.75 and 3.0 mg of propolis/ml, However, the two samples of propolis showed different levels of inhibition on each of the enzymes tested. in general, EEP RS demonstrated a significantly higher inhibitory activity on GtfB and C activities (both solution and surface assays) than EEP MG at concentrations between 0.047 and 0.187 mg/ml (p<0.05), EEP MG, on the other hand, exhibited a greater inhibitory effect on the activities of surface GtfD (at 0.375, 0.75 and 1.5 mg/ml) and S. sanguis Gtf (at 1.5 and 3.0 mg/ml; p<0.05). These data indicate that EEP is a potent inhibitor of Gtf enzymes in solution and adsorbed on an experimental pellicle; however, its effect on Gtf activity is variable depending on the geographical origin of the propolis samples. There is a need to identify the active compounds of propolispt
dc.relation.ispartofCaries researchpt_BR
dc.relation.ispartofabbreviationCaries res.pt_BR
dc.publisher.cityBaselpt_BR
dc.publisher.countrySuiçapt_BR
dc.publisherKargerpt_BR
dc.date.issued2000pt_BR
dc.date.monthofcirculationSept./Oct.pt_BR
dc.identifier.citationCaries Research. Karger, v. 34, n. 5, n. 418, n. 426, 2000.pt_BR
dc.language.isoengpt_BR
dc.description.volume34pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage418pt_BR
dc.description.lastpage426pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0008-6568pt_BR
dc.identifier.eissn1421-976Xpt_BR
dc.identifier.doi10.1159/000016617pt_BR
dc.identifier.urlhttps://www.karger.com/Article/Abstract/16617pt_BR
dc.description.sponsorshipCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORpt_BR
dc.description.sponsorship1CAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORpt_BR
dc.description.sponsordocumentnumber0574/98-20pt_BR
dc.date.available2014-12-02T16:30:43Z
dc.date.available2015-11-26T17:40:25Z-
dc.date.accessioned2014-12-02T16:30:43Z
dc.date.accessioned2015-11-26T17:40:25Z-
dc.description.provenanceMade available in DSpace on 2014-12-02T16:30:43Z (GMT). No. of bitstreams: 1 WOS000089280300008.pdf: 297174 bytes, checksum: 70826bc78e2f019798dca55b31a6944d (MD5) Previous issue date: 2000 Bitstreams deleted on 2020-05-14T20:38:33Z: WOS000089280300008.pdf,. Added 1 bitstream(s) on 2020-05-14T20:42:47Z : No. of bitstreams: 2 000089280300008.pdf: 473724 bytes, checksum: 5d05a35b6e715f19306a7981a6ef9e45 (MD5) WOS000089280300008.pdf.txt: 38684 bytes, checksum: 87e3cb1fec15efa7bcec32a6d93d35f8 (MD5) Bitstreams deleted on 2020-06-04T12:47:37Z: 000089280300008.pdf,. Added 1 bitstream(s) on 2020-06-04T12:52:46Z : No. of bitstreams: 2 000089280300008.pdf: 774608 bytes, checksum: 814836bd1fe835116e63f424755c10f4 (MD5) WOS000089280300008.pdf.txt: 38684 bytes, checksum: 87e3cb1fec15efa7bcec32a6d93d35f8 (MD5)en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:40:25Z (GMT). No. of bitstreams: 2 WOS000089280300008.pdf: 297174 bytes, checksum: 70826bc78e2f019798dca55b31a6944d (MD5) WOS000089280300008.pdf.txt: 38684 bytes, checksum: 87e3cb1fec15efa7bcec32a6d93d35f8 (MD5) Previous issue date: 2000en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64085pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/64085
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64085-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Ciências Fisiológicaspt_BR
dc.contributor.departmentDepartamento de Ciências Fisiológicaspt_BR
dc.contributor.departmentDepartamento de Ciência de Alimentospt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Engenharia de Alimentospt_BR
dc.subject.keywordGlucosyltransferasept_BR
dc.subject.keywordStreptococcipt_BR
dc.subject.keywordPlaquept_BR
dc.subject.keywordGlucanpt_BR
dc.identifier.source000089280300008-
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0003-0812-4027pt_BR
dc.creator.orcid0000-0003-1046-5605pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.type.formArtigopt_BR
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