Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDual gene transfer of fibroblast growth factor-2 and platelet derived growth factor-BB using plasmid deoxyribonucleic acid promotes effective angiogenesis and arteriogenesis in a rodent model of hindlimb ischemiapt_BR
dc.contributor.authorDe Paula, EVpt_BR
dc.contributor.authorFlores-Nascimento, MCpt_BR
dc.contributor.authorArruda, VRpt_BR
dc.contributor.authorGarcia, RApt_BR
dc.contributor.authorRamos, CDpt_BR
dc.contributor.authorGuillaumon, ATpt_BR
dc.contributor.authorAnnichino-Bizzacchi, JMpt_BR
unicamp.authorDe Paula, Erich V. Univ Estadual Campinas, Hematol & Hemotherapy Ctr, BR-13083970 Campinas, SP, Brazil Univ Penn, Childrens Hosp Philadelphia, Sch Med, Philadelphia, PA 19104 USA Univ Estadual Campinas, Fac Med Sci, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subject.wosRat Modelpt_BR
dc.description.abstractThe protein infusion of basic fibroblast growth factor-2 (FGF-2) and platelet derived growth factor-BB (PDGF-BB) have been shown to promote the formation of a stable and functional vascular network in small and large animal models of ischemia. Here, we sought to determine whether a similar effect could be obtained using a gene-therapy-based strategy with nonviral vectors. Rats underwent a surgical procedure to create hindlimb ischemia and were injected with a combination of plasmids that expressed FGF-2 and PDGF-BB. Anatomical and functional parameters of the angiogenesis and arteriogenesis response were evaluated after 4 weeks. The results were compared with rats injected with plasmids that expressed a reporter gene or the extensively studied vascular endothelial growth factor (VEGF165) alone. Treatment with the FGF-2/PDGF-BB combination increased the angiogenesis and arteriogenesis response compared with the empty plasmid, and it was as effective as VEGF165. In terms of safety, the combination allowed the use of a 50% lower individual dose of each plasmid and in addition promoted the formation of more stable vessels than VEGF165. In conclusion, the dual gene transfer of FGF-2 and PDGF-BB using nonviral vectors is safe and effective in promoting the formation of a functional vascular network in a rodent model of hindlimb ischemia. (Translational Research 2009; 153:232-239)pt
dc.relation.ispartofTranslational Researchpt_BR
dc.relation.ispartofabbreviationTransl. Res.pt_BR
dc.publisher.cityNew Yorkpt_BR
dc.publisherElsevier Science Incpt_BR
dc.identifier.citationTranslational Research. Elsevier Science Inc, v. 153, n. 5, n. 232, n. 239, 2009.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsordocumentnumberFAPESP [2004-13158-2]pt
dc.description.provenanceMade available in DSpace on 2014-11-16T01:45:30Z (GMT). No. of bitstreams: 1 WOS000265756600004.pdf: 457946 bytes, checksum: e7c0f58e6e73cabac2854933847b7460 (MD5) Previous issue date: 2009en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:37:10Z (GMT). No. of bitstreams: 2 WOS000265756600004.pdf: 457946 bytes, checksum: e7c0f58e6e73cabac2854933847b7460 (MD5) WOS000265756600004.pdf.txt: 31756 bytes, checksum: 2a2e926ead79944d5c2850f0f068ea3d (MD5) Previous issue date: 2009en
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000265756600004.pdf447.21 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.