Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/633
Type: Artigo de periódico
Title: Bovine pericardium coated with biopolymeric films as an alternative to prevent calcification: In vitro calcification and cytotoxicity results
Author: NOGUEIRA, Grinia M.
RODAS, Andrea C. D.
WESKA, Raquel F.
AIMOLI, Cassiano G.
HIGA, Olga Z.
MAIZATO, Marina
LEINER, Adolfo A.
PITOMBO, Ronaldo N. M.
POLAKIEWICZ, Bronislaw
BEPPU, Marisa M.
Abstract: Bovine pericardium, for cardiac valve fabrication, was coated with either chitosan or silk fibroin film. In vitro calcification tests of coated and non coated bovine pericardium were performed in simulated body fluid solution in order to investigate potential alternatives to minimize calcification on implanted heart valves. Complementary, morphology was assessed by scanning electron microscopy - SEM; X-ray diffraction (XRD) and infrared spectroscopy (FTIR-ATR) were performed for structural characterization of coatings and biocompatibility of chitosan. Silk fibroin films were assayed by in vitro cytotoxicity and endothelial cell growth tests. Bovine pericardium coated with silk fibroin or chitosan did not present calcification during in vitro calcification tests, indicating that these biopolymeric coatings do not induce bovine pericardium calcification. Chitosan and silk fibroin films were characterized as non cytotoxic and silk fibroin films presented high affinity to endothelial cells. The results indicate that bovine pericardium coated with silk fibroin is a potential candidate for cardiac valve fabrication, since the affinity of silk fibroin to endothelial cells can be explored to induce the tissue endothelization and therefore, increase valve durability by increasing their mechanical resistance and protecting them against calcification. (C) 2010 Elsevier B.V. All rights reserved.
Subject: In vitro calcification
Bovine pericardium
Silk fibroin
Chitosan
Cardiac valves
Cytotoxicity
Country: Holanda
Editor: ELSEVIER SCIENCE BV
Citation: Materials science and engineering. C, Biomimetic materials, sensors and systems, v.30, n.4, p.575-582, 2010
Rights: fechado
Identifier DOI: 10.1016/j.msec.2010.02.011
Address: http://dx.doi.org/10.1016/j.msec.2010.02.011
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Date Issue: 2010
Appears in Collections:FEQ - Artigos e Outros Documentos

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