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|Title:||Discrimination of the nucleolus by a critical electrolyte concentration method|
|Author:||Mello, Maria Luiza S.|
Vidal, Benedicto de Campos
Dantas, Marly M.
Monteiro, Ana L. P.
|Abstract:||A new technique for the visualization of the nucleolus based on a variant of the previously reported critical electrolyte concentration method for nucleoprotein complexes is proposed. The method consists of treating toluidine blue-stained preparations with Mg2+ ions in a concentration such that ribonucleoprotein complexes are metachromatically highlighted, while DNA-protein complexes attain their critical electrolyte concentration point, thus losing metachromasy. The technique, which is applicable to plant and animal cells, is proposed for studies involving identification, morphometry and eventually changes in substructure of the nucleolus at the light microscopy level.|
Azul de toluidina
|Editor:||Japan Society of Histochemistry and Cytochemistry|
|Citation:||Acta Histochemica Et Cytochemica. Japan Soc Histochemistry & Cytochemistry, v. 26, n. 1, n. 1, n. 3, 1993.|
|Appears in Collections:||IB - Artigos e Outros Documentos|
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