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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDot-enzyme linked immunosorbent assay as an alternative technique for the detection of bovine respiratory syncytial virus (BRSV) antibodiespt_BR
dc.contributor.authorDomingues, HGpt_BR
dc.contributor.authorCampalans, Jpt_BR
dc.contributor.authorAlmeida, RSpt_BR
dc.contributor.authorCoswig, LTpt_BR
dc.contributor.authorArns, CWpt_BR
unicamp.authorUniv Estadual Campinas, UNICAMP, Inst Biol, Dept Microbiol & Imunol,Lab Virol Anim, BR-13081970 Campinas, SP, Brazilpt_BR
dc.subjectbovine respiratory syncytial viruspt_BR
dc.subjectcommercial ELISA kitpt_BR
dc.subjectserological analysispt_BR
dc.description.abstractA dot-ELISA test for the detection of anti-BRSV antibodies is described. The objective of this study was the standardisation of a test as a fast, inexpensive and effective alternative to detect anti-BRSV antibodies. Its sensitivity, specificity and usefulness were compared to a commercial ELISA-kit and to the standard serum neutralisation (SN) test. The standardisation of the technique was done using nitrocellulose disks soaked with a viral sample isolated in Brazil, BRSV-25-BR. The best results were obtained when the disks were sensitised with a purified antigen at a concentration of 0.7 mug/disk and the bovine serum was diluted 1:200. The experiment used 423 samples of bovine serum collected in the main cattle breeding centres in Brazil. The standard SN, dot-ELISA technique and commercial ELISA kits scored 67.8%, 71.8% and 72.3% of the samples as positive, respectively. When compared to the SN test, the standardised dot-ELISA and the commercial ELISA tests presented relative sensitivities of 92.3% and 91.6% and relative specificities of 71.3% and 68.4% respectively. The results demonstrated that the dot-ELISA test is adequate for the objectives proposed by this study, being easy to use and economically viable. Thus, this test represents an alternative for BRSV serological diagnosis in the substitution of SN and commercial ELISA tests, recommendable for utilisation in laboratories with few
dc.relation.ispartofVeterinary Researchpt_BR
dc.relation.ispartofabbreviationVet. Res.pt_BR
dc.publisher.cityLes Ulis Cedexapt_BR
dc.publisherE D P Sciencespt_BR
dc.identifier.citationVeterinary Research. E D P Sciences, v. 33, n. 4, n. 397, n. 404, 2002.pt_BR
dc.sourceWeb of Sciencept_BR
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dc.description.provenanceMade available in DSpace on 2015-11-26T17:12:27Z (GMT). No. of bitstreams: 2 WOS000177116000006.pdf: 197533 bytes, checksum: 951b79da1dfb5bee5ab2e3634ca57754 (MD5) WOS000177116000006.pdf.txt: 23434 bytes, checksum: 980cf393b1206123ca4183a4b4a0f577 (MD5) Previous issue date: 2002en
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