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Type: Artigo de periódico
Title: Determination of RSD921 in human plasma by high-performance liquid chromatography-tandem mass spectrometry using tri-deuterated RSD921 as internal standard: application to a phase I clinical trial
Author: Ribeiro, W
Ifa, DR
Corso, G
Salmon, J
Moraes, LA
Eberlin, MN
de Nucci, G
Abstract: A fast, sensitive and specific method is presented for the quantification of RSD921 in human plasma by liquid chromatography coupled with tandem mass spectrometry using tri-deuterated RSD921 (3d-RSD921) as an internal standard. A single-step liquid/liquid extraction was performed with diethyl ether/hexane (80:20, v/v) using 0.5 ml of plasma. The plasma calibration curves were linear from 0.1 to 20 ng ml(-1) (r > 0.999). Between-run precision, based on the percent relative deviation for replicate (n = 40) quality controls, was less than or equal to 7.27% (0.5 ng ml(-1)), less than or equal to 7.39% (5.0 ng ml(-1)), and less than or equal to 5.06% (20.0 ng ml(-1)). Between-run accuracies, based on the relative error, were +/-2.59%, +/-1.23% and +/-1.64% respectively. The method was developed to evaluate the pharmacokinetic profile after 15 min of intravenous stepwise-ascending infusion dose of RSD921 in IS healthy volunteers. A dissociation study of protonated RSD921 and 3d-RSD921 by collision-induced dissociation using in-source fragmentation and tandem mass spectrometry is also presented. Copyright (C) 2001 John Wiley & Sons, Ltd.
Subject: arylacetamide
Country: Inglaterra
Editor: John Wiley & Sons Ltd
Citation: Journal Of Mass Spectrometry. John Wiley & Sons Ltd, v. 36, n. 10, n. 1133, n. 1139, 2001.
Rights: fechado
Identifier DOI: 10.1002/jms.218
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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