Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

Abstract

Strongyloides stercoralis causes chronic asymptomatic infections in immunocompetent human hosts and systemic invasion in immunocompromised patients developing into a fatal hyperinfection syndrome IgA and IgG detection in saliva and serum paired samples were tested using total saline extract from Strongyloides venezuelensis (SE) and its detergent phase (D) extracted with Triton X-114 Saliva and serum paired samples were obtained from 25 patients with confirmed strongyloidiasis 25 patients with other parasitoses and 20 from apparently healthy individuals Sensitivity specificity diagnostic efficiency positive and negative predictive values and likelihood ratio were calculated at the optimum point of reaction Using D phase sensitivity and specificity to detect IgA in saliva were 76 0% and 88 9% and in serum 80 0% and 86 7% respectively To detect IgG D phase showed sensitivity and specificity of 88 0% and 88 9% in saliva and 88 0% and 84 4% in serum respectively D phase proved to be specific and efficient and could be utilized as an alternative antigen for IgA and IgG detection in saliva and serum samples for strongyloidiasis diagnosis (C) 2010 Elsevier B V All rights reserved