Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Ion mobility spectrometry focusing on speciation analysis of metals/metalloids bound to carbonic anhydrase
Author: Pessoa, GD
Pilau, EJ
Gozzo, FC
Arruda, MAZ
Abstract: In the present work, traveling wave ion mobility spectrometry-mass spectrometry (TWIMS-MS) was applied to speciation analysis of metalloproteins. The influence of pH on complexation conditions between some metals and bovine carbonic anhydrase was evaluated from pH 6 to 9, as well as the time involved in their complexation (0-24 h). Employing TWIMS-MS, two conformational states of bovine carbonic anhydrase were observed with charge states of +12 and +11; these configurations being evaluated in terms of the folded state of the apo form and this protein (at charge state +11) being linked to barium, lead, copper, and zinc in their divalent forms. Metalloprotein speciation analysis was carried out for copper (Cu+ and Cu2+), lead (Pb2+ and Pb4+), and selenium (Se4+ and Se6+) species complexed with bovine carbonic anhydrase. Mobilities of all complexed species were compared, also considering the apo form of this protein.
Subject: Bioanalytical methods
Mass spectrometry
Country: Alemanha
Editor: Springer Heidelberg
Citation: Analytical And Bioanalytical Chemistry. Springer Heidelberg, v. 405, n. 24, n. 7653, n. 7660, 2013.
Rights: fechado
Identifier DOI: 10.1007/s00216-013-7064-1
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.