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Type: Artigo de periódico
Title: Inflammatory and bone regulators expression in murine macrophages under exposure of commercial and experimental mineral trioxide aggregate
Author: Pereda, GO
Fudinaga, ACC
Beltran, HS
Peroni, LA
Stach-Machado, D
Abstract: Background: Mineral trioxide aggregate (MTA) has been used in a variety of surgical and non-surgical endodontic applications. The aim of this study was to evaluate the gene expression and protein production of TNF-alpha, IL-1 beta and IL-6, as well as the gene expression of RANKL and OPG using both commercial and experimental MTA in macrophage cell cultures. Methods: Peritoneal macrophage cell culture was performed. Viability, gene expression of cytokines, RANKL and OPG, and protein levels in experimental- and commercial-grey MTA co-cultured with peritoneal macrophages was determined by tryptan blue, real time PCR and ELISA. Results: The expression of TNF-alpha for both commercial and experimental MTA was higher, while the expression of IL-1 beta and IL-6 was similar when compared to the negative control. At protein expression level, no differences were observed between the negative control and cements. RANKL did not show a significant improvement in gene expression when compared with the negative control, but OPG expression in cement samples was higher when compared to the negative control. Conclusions: This study suggests that commercial and experimental MTA promotes anti-inflammatory processes, as well as bone healing capacity.
Subject: Mineral trioxide aggregates
Country: EUA
Editor: Wiley-blackwell
Citation: Australian Dental Journal. Wiley-blackwell, v. 57, n. 3, n. 284, n. 291, 2012.
Rights: fechado
Identifier DOI: 10.1111/j.1834-7819.2012.01701.x
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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