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Type: Artigo de periódico
Title: Purification, crystallization and preliminary crystallographic study of a Kunitz-type trypsin inhibitor from Delonix regia seeds
Author: Polikarpov, I
Golubev, AM
Perles, LA
Pando, SC
Novello, JC
Marangoni, S
Abstract: The Kunitz-type trypsin inhibitor from seeds of Flamboyant (Delonix regia) has been purified to homogeneity and plate-like crystals suitable for X-ray analysis have been grown by the hanging-drop method using PEG 6000 as a precipitant. The crystals belong to space group P2(1)2(1)2(1) With unit-cell parameters a = 32.15, b = 69.39, c = 72.54 Angstrom. X-ray diffraction data have been collected to 2.95 A resolution. The structure has been solved by molecular replacement using the known structures of trypsin inhibitors from Erythrina caffra seeds (PDB code 1tie) and from soya beans (Glycine max; PDB code 1ba7) as search models.
Country: Dinamarca
Editor: Munksgaard Int Publ Ltd
Citation: Acta Crystallographica Section D-biological Crystallography. Munksgaard Int Publ Ltd, v. 55, n. 1611, n. 1613, 1999.
Rights: aberto
Identifier DOI: 10.1107/S0907444999009361
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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