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Type: Artigo de periódico
Title: Purification, characterization and application of laccase from Trametes versicolor for colour and phenolic removal of olive mill wastewater in the presence of 1-hydroxybenzotriazole
Author: Minussi, RC
Miranda, MA
Silva, JA
Ferreira, CV
Aoyama, H
Marangoni, S
Rotilio, D
Pastore, GM
Duran, N
Abstract: Laccase forms ( L1 and L2) from Trametes versicolor CCT 4521 showed a molecular mass of 66 kDa and optimum temperature around 40 degrees C. The optimum pH ( 4.0 and 5.0) and K-m ( 28.6 and 5 mu M) values using syringaldazine as substrate were found for L1 and L2, respectively. The enzymes were able to oxidize several compounds and were strongly inhibited by sodium azide, L-cysteine and dithiothreitol. The 75% of the N-terminal sequences were identical in both forms and similarities around 40-60% of laccases from wood-degrading fungi were observed. The use of 1-hydroxybenzotriazole as a mediator increased the compounds oxidized by laccases in olive mill wastewater.
Subject: laccase
Trametes versicolor
olive mill wastewater
Editor: Academic Journals
Citation: African Journal Of Biotechnology. Academic Journals, v. 6, n. 10, n. 1248, n. 1254, 2007.
Rights: aberto
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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