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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titlePurification of triolein for use in semipermeable membrane devices (SPMDs)pt_BR
dc.contributor.authorLebo, JApt_BR
dc.contributor.authorAlmeida, FVpt_BR
dc.contributor.authorCranor, WLpt_BR
dc.contributor.authorPetty, JDpt_BR
dc.contributor.authorHuckins, JNpt_BR
dc.contributor.authorRastall, Apt_BR
dc.contributor.authorAlvarez, DApt_BR
dc.contributor.authorMogensen, BBpt_BR
dc.contributor.authorJohnson, BTpt_BR
unicamp.authorUSGS, CERC, Columbia, MO 65201 USA Univ Estadual Campinas, Inst Quim, Campinas, SP, Brazil Chem Lab, Inst Hyg, INF 324, Heidelberg, Germany Natl Environm Res Inst, Dept Environm Chem, DK-4000 Roskilde, Denmarkpt_BR
dc.subjectoleic acidpt_BR
dc.subjectmethyl oleatept_BR
dc.subjectdialysispt_BR
dc.subjectpurificationpt_BR
dc.subjectMicrotoxpt_BR
dc.subjectyeast estrogen screenpt_BR
dc.subjectYESpt_BR
dc.subject.wosAromatic-hydrocarbonspt_BR
dc.subject.wosContaminantspt_BR
dc.subject.wosAirpt_BR
dc.description.abstractAnalyses of triolein-containing semipermeable membrane devices (SPMDs) have sometimes been impeded by interferences caused by impurities endemic to triolein that codialyze with the analytes. Oleic acid and methyl oleate have been the most troublesome of these impurities because of their relatively high concentrations in triolein and because significant residues of both can persist even after size exclusion chromatographic (SEC) fractionation. These residues have also been blamed for false-positive signals during bioindicator testing of SPMD dialysates. To prevent these problems, a simple, cost-effective procedure was developed for purifying triolein destined for use in SPMDs: the bulk triolein is repeatedly (6x) partitioned against methanol. Tests of the procedure show that C-14-oleic acid is completely removed from the triolein. After SEC fractionation, dialysates of standard-size SPMDs made with the purified triolein contain less than 5 mug of methyl oleate as compared to sometimes more than 500 mug for dialysates (also after SEC) of SPMDs made with unpurified triolein. Gas chromatographic analyses with flame ionization and electron capture detection show that the purification treatment also greatly reduces the number and size of peaks caused by unidentified contaminants in the triolein. Microtox basic assay of dialysates of SPMDs shows that those made with the purified triolein have lower acute toxicities than dialysates of SPMDs made with unpurified triolein. Yeast estrogen screen (YES) testing of SPMDs fabricated with unpurified and purified triolein demonstrates that the purification process removes all background estrogenic activity. Published by Elsevier Ltd.pt
dc.relation.ispartofChemospherept_BR
dc.relation.ispartofabbreviationChemospherept_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryInglaterrapt_BR
dc.publisherPergamon-elsevier Science Ltdpt_BR
dc.date.issued2004pt_BR
dc.date.monthofcirculationFEBpt_BR
dc.identifier.citationChemosphere. Pergamon-elsevier Science Ltd, v. 54, n. 8, n. 1217, n. 1224, 2004.pt_BR
dc.language.isoenpt_BR
dc.description.volume54pt_BR
dc.description.issuenumber8pt_BR
dc.description.firstpage1217pt_BR
dc.description.lastpage1224pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0045-6535pt_BR
dc.identifier.wosidWOS:000187802900021pt_BR
dc.identifier.doi10.1016/j.chemosphere.2003.09.014pt_BR
dc.date.available2014-11-16T17:39:56Z
dc.date.available2015-11-26T16:24:31Z-
dc.date.accessioned2014-11-16T17:39:56Z
dc.date.accessioned2015-11-26T16:24:31Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:24:31Z (GMT). No. of bitstreams: 2 WOS000187802900021.pdf: 280217 bytes, checksum: 0ff815802e99361de8c6c972ea68d442 (MD5) WOS000187802900021.pdf.txt: 37119 bytes, checksum: ff7c36b9bf2f7b382b9f21e565992991 (MD5) Previous issue date: 2004en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58485pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/58485
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/58485-
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