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Type: Artigo de periódico
Title: Purification and biological effects of Araucaria angustifolia (Araucariaceae) seed lectin
Author: Santi-Gadelha, T
Gadelha, CADA
Aragao, KS
de Oliveira, CC
Mota, MRL
Gomes, RC
Pires, ADF
Toyama, MH
Toyama, DDO
de Alencar, NMN
Criddle, DN
Assreuy, AMS
Cavada, BS
Abstract: This paper describes the purification and characterization of a new N-acetyl-D-glucosamine-specific lectin from Araucaria angustifolia (AaL) seeds (Araucariaceae) and its anti-inflammatory and antibacterial activities. AaL was purified using a combination of affinity chromatography on a chitin column and ion exchange chromatography on Sephacel-DEAE. The pure protein has 8.0 kDa (SDS-PAGE) and specifically agglutinates rabbit erythrocytes, effect that was independent of the presence of divalent cations and was inhibited after incubation with glucose and N-acetyl-D-glucosamine. AaL showed antibacterial activity against Gram-negative and Gram-positive strains, shown by scanning electron microscopy. AaL, intravenously injected into rats, showed anti-inflammatory effect, via carbohydrate site interaction, in the models of paw edema and peritonitis. This lectin can be used as a tool for studying bacterial infections and inflammatory processes. (c) 2006 Elsevier Inc. All rights reserved.
Subject: plant lectin seeds
Araucaria angustifolia
Country: EUA
Editor: Academic Press Inc Elsevier Science
Citation: Biochemical And Biophysical Research Communications. Academic Press Inc Elsevier Science, v. 350, n. 4, n. 1050, n. 1055, 2006.
Rights: fechado
Identifier DOI: 10.1016/j.bbrc.2006.09.149
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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